种鸽场沙门菌的分离鉴定及耐药性分析  

Isolation,Identification and Antimicrobial Resistance Analysis of Salmonella Strains from Pigeon Breeding Farm

作　　者：张明霞 王鹏[1] 黄振兴 秦雨欣 廖巧怡 周国宇 韦天超[1,2] ZHANG Mingxia;WANG Peng;HUANG Zhenxing;QIN Yuxin;LIAO Qiaoyi;ZHOU Guoyu;WEI Tianchao(College of Animal Science and Technology,Guangxi University,Nanning 530004,China;Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics,Nanning 530004,China)

机构地区：[1]广西大学动物科学技术学院,广西南宁530004 [2]广西壮族自治区兽用生物制品工程研究中心,广西南宁530004

出　　处：《畜牧与饲料科学》2024年第5期117-122,共6页Animal Husbandry and Feed Science

基　　金：广西重点研发计划项目(桂科AB23026082);广西大学“大学生创新创业训练计划”(S202210593218);校企合作项目(202201277)。

摘　　要：[目的]了解广西壮族自治区种鸽场鸽群沙门菌感染情况及流行菌株的耐药性特征,为该地区鸽沙门菌病的防治提供参考。[方法]从广西壮族自治区南宁市某种鸽场疑似感染沙门菌的病(死)鸽采集肝脏组织样本,进行沙门菌的分离和纯化培养;采用革兰染色镜检、生化鉴定、血清型鉴定方法对分离菌株进行表型鉴定;应用PCR方法扩增沙门菌invA基因,对分离菌株进行分子生物学鉴定;利用K-B纸片法对分离菌株进行药物敏感性试验。[结果]根据菌落生长特征、革兰染色特性和细菌形态以及血清型鉴定、生化鉴定、分子生物学鉴定结果,从发病鸽场分离与鉴定了2株鼠伤寒沙门菌(Salmonella typhimurium),分别命名为S1株和S2株。2株沙门菌分离株均对阿莫西林、链霉素、林可霉素和萘啶酸呈现耐药性,对红霉素、头孢噻吩和利福平中度敏感,对环丙沙星、复方新诺明、氯霉素和甲氧苄啶敏感。[结论]从南宁市某种鸽场病(死)鸽临床样本中分离鉴定了2株鼠伤寒沙门菌,菌株对部分抗菌药物表现出较强耐药性,对该地区种鸽健康养殖存在潜在威胁,提示应加强该地区种鸽场沙门菌耐药性的检测。[Objective]The aims of the present study were to characterize the prevalence and antimicrobial resistance pattern of Salmonella strains in pigeon flock of a pigeon breeding farm in Guangxi Zhuang Autonomous Region,and to provide references for the prevention and treatment of pigeon salmonellosis in this area.[Method]The clinical samples of liver were collected from the diseased or dead pigeons with suspected Salmonella infection in a pigeon breeding farm in Nanning City,Guangxi Zhuang Autonomous Region,and were subjected to isolation and culture of Salmonella strains.Phenotypic identification of the isolates was performed by Gram staining and microscopy,biochemical identification and serotype identification.Subsequently,the isolates were molecularly confirmed by PCR assay targeting invA gene specific for Salmonella.The antimicrobial susceptibility test of the isolates was conducted by Kirby-Bauer disk diffusion method.[Result]Two strains of Salmonella typhimurium,designated as S1 and S2,were isolated and identified from the clinical samples according to colony growth features,Gram staining characteristics,bacterial morphology,serotype identification,biochemical identification and molecular comfirmation.Both isolates were resistant to amoxicillin,streptomycin,lincomycin and nalidixic acid,moderately sensitive to erythromycin,cefthiophene and rifampicin,and sensitive to ciprofloxacin,cotrimoxazole,chloramphenicol and trimethoprim.[Conclusion]Two strains of Salmonella typhimurium were obtained from the clinical samples of the diseased or dead pigeons in a pigeon breeding farm in Nanning City,and the isolates exhibited high level resistance to certain antimicrobial agents,posing a potential threat to the health of breeding pigeon flocks in this area.It was recommended that detecting the prevalence of antimicrobial resistant Salmonella should be strengthened.

关 键 词：鸽 沙门菌 分离鉴定 耐药性 

分 类 号：S858.39[农业科学—临床兽医学] S852.612[农业科学—兽医学]