子痫前期患者血清lncRNA HIF1A-AS1,CXCL9水平表达与病情程度及不良妊娠结局的关系分析  

Analysis of Relationship between Serum lncRNA HIF1A-AS1,CXCL9 and Disease Severity,Adverse Pregnancy Outcomes in Patients with Preeclampsia

作　　者：张金辉 李维玲[1] 闫璐 ZHANG Jinhui;LI Weiling;YAN Lu(Department of Gynaecology and Obstetrics,Xi’an Gaoxin Hospital,Xi’an 710075,China)

机构地区：[1]西安高新医院妇产科,西安710075

出　　处：《现代检验医学杂志》2024年第6期73-78,140,共7页Journal of Modern Laboratory Medicine

基　　金：陕西省科技计划项目(编号:2020SF-344)。

摘　　要：目的探讨子痫前期(Preeclanpsia)患者血清长链非编码RNA缺氧诱导因子-1α-反义链1(lncRNA HIF1A-AS1)、CXC趋化因子配体9(CXCL9)表达与病情程度及不良妊娠结局的关系。方法以2021年1月~2022年12月西安高新医院收治的83例子痫前期患者为子痫前期组,根据确诊时间对子痫前期患者进行分组,分为早发型子痫前期组(n=39)和晚发型子痫前期组(n=44);根据病情严重程度将子痫前期患者分为轻度子痫前期组(n=51)和重度子痫前期组(n=32);根据妊娠结局分为正常妊娠结局组(n=56)和不良妊娠结局组(n=27)。收集同期的60例健康妊娠孕妇为对照组。采用实时荧光定量PCR(qRT-PCR)检测血清lncRNA HIF1A-AS1相对表达量,酶联免疫吸附法(ELISA)检测血清CXCL9水平。Spearman秩相关分析血清lncRNA HIF1A-AS1,CXCL9与病情程度的相关性,Logistic回归分析子痫前期患者不良妊娠结局影响因素,绘制受试者工作特征(ROC)曲线评估血清lncRNA HIF1A-AS1,CXCL9对不良妊娠结局预测价值。结果子痫前期组血清lncRNA HIF1A-AS1相对表达量(0.73±0.26)低于对照组(1.15±0.34),CXCL9水平(209.34±45.34 pg/ml)高于对照组(116.80±37.76 pg/ml),差异具有统计学意义(t=8.379,12.903,均P<0.05)。早发型子痫前期组血清lncRNA HIF1A-AS1相对表达量(0.58±0.21)低于晚发型子痫前期组(0.86±0.27),CXCL9水平(236.60±31.02 pg/ml)高于对照组(185.18±23.63 pg/ml),差异具有统计学意义(t=5.226,8.551,均P<0.05)。重度子痫前期组血清lncRNA HIF1A-AS1相对表达量(0.52±0.21)低于轻度子痫前期组(0.97±0.34),血清CXCL9水平(253.38±41.20 pg/ml)高于轻度子痫前期组(159.65±40.79 pg/ml),差异具有统计学意义(t=6.409,10.152,均P<0.05)。血清lncRNA HIF1A-AS1,CXCL9与子痫前期病情具有相关性(r=-0.627,0.651,均P<0.05)。CXCL9[OR(95%CI):1.581(1.098~2.276)]是子痫前期不良妊娠结局独立危险因素(P<0.05),lncRNA HIF1A-AS1[OR(95%CI):0.806(0.673~0.965)]是保护因素(P<0.05);早发型Objective To explore the relationship between serum long non-coding RNA hypoxia-inducible factor 1 alpha antisense RNA 1(lncRNA HIFA1-AS1),CXC chemokine ligand 9(CXCL9)and disease severity,adverse pregnancy outcome in patients with preeclampsia.Methods A total of 83 patients with preeclampsia admitted to Xi'an Gaoxin Hospital January 2021 to December 2022 were taken as preeclampsia group,According to the time of diagnosis,patients with preeclampsia were divided into early onset preeclampsia group(n=39)and late onset preeclampsia group(n=44).According to the severity of the condition,patients with preeclampsia were divided into two groups:mild preeclampsia group(n=51)and severe preeclampsia group(n=32).According to pregnancy outcomes,they were divided into a normal pregnancy outcome group(n=56)and an adverse pregnancy outcome group(n=27).At the same time,60 healthy pregnant women with pregnancy were collected as control group.Real-timefluorescence quantitative PC(qRT-PCR)was used to detect serum lncRNA HIF1A-AS1,and enzyme-linked immunosorbent assay(ELISA)was used to detect serum CXCL9.Spearman rank correlation analysis was used to analyze the association between serum lncRNA HIF1A-AS1,CXCL9 and severity of disease.Logistic regression analysis was used to analyze the factors influencing the adverse pregnancy outcome in patients with preeclampsia.ROC curve was drawn to assess the predictive value of serum lncRNA HIF1A-AS1,CXCL9 for adverse pregnancy outcome.Results The relative expression of serum lncRNA HIF1A-AS1(0.73±0.26)in preeclampsia group was lower than that(1.15±0.34)in control group,and CXCL9 level(209.34±45.34 pg/ml)higher than that(116.80±37.76 pg/ml)in control group,the differences were statistically significant(t=8.379,12.903,all P<0.05).The relative expression of serum lncRNA HIF1A-AS1(0.58±0.21)in early-onset preeclampsia group was lower than that in late-onset preeclampsia group(0.86±0.27),and CXCL9 level(236.60±31.02 pg/ml)higher than that in late-onset preeclampsia group(185.18±23.63 pg/

关 键 词：子痫前期 长链非编码RNA缺氧诱导因子-1α-反义链1 CXC趋化因子配体9 不良妊娠结局 

分 类 号：R714.244[医药卫生—妇产科学] R392.11[医药卫生—临床医学]