持续性心房颤动患者血清miR-133a-3p和miR-324-3p表达与射频消融术后房颤复发的关系  

Relationship between the Expressions of Serum miR-133a-3p and miR-324-3p in Patients with Persistent Atrial Fibrillation and the Recurrence of Atrial Fibrillation after Radiofrequency Ablation

作　　者：王岳胜 刘晓晨 刘恩香 李晶晶 WANG Yuesheng;LIU Xiaochen;LIU Enxiang;LI Jingjing(Department of Cardiology,Cangzhou People’s Hospital,Hebei Cangzhou 061000,China;Heart Center,Cangzhou People’s Hospital,Hebei Cangzhou 061000,China)

机构地区：[1]沧州市人民医院心内科,河北沧州061000 [2]沧州市人民医院心脏中心,河北沧州061000

出　　处：《现代检验医学杂志》2024年第6期108-112,118,共6页Journal of Modern Laboratory Medicine

基　　金：河北省卫生健康委科研基金项目(20200292)。

摘　　要：目的探讨血清miR-133a-3p、miR-324-3p在持续性心房颤动患者血清中的表达以及与射频消融术后房颤复发的关系。方法收集2019年7月~2022年7月于沧州市人民医院住院诊治并接受射频消融术治疗的180例持续性心房颤动患者(持续性心房颤动组)作为研究对象,根据房颤是否复发,分为无复发组(n=116)和复发组(n=64),另选取同期在该院体检的180例健康人作为对照组。比较各组血清miR-133a-3p,miR-324-3p表达水平;多因素Logistic回归分析持续性心房颤动患者射频消融术后房颤复发的影响因素;ROC曲线分析血清miR-133a-3p,miR-324-3p水平对持续性心房颤动患者射频消融术后房颤复发的预测价值。结果持续性心房颤动组血清miR-133a-3p(0.76±0.25),miR-324-3p(0.68±0.21)水平显著低于对照组(1.03±0.32,1.05±0.30),差异具有统计学意义(t=8.921,13.556,均P<0.05)。复发组血清miR-133a-3p(0.58±0.19),miR-324-3p水平(0.50±0.16)均显著低于无复发组(0.86±0.27,0.78±0.25),差异具有统计学意义(t=7.349,8.087,均P<0.055)。多因素Logistic回归分析,结果显示,血清miR-133a-3p[OR(95%CI):0.673(0.534~0.848)],miR-324-3p[OR(95%CI):0.756(0.629~0.909)]为持续性心房颤动患者射频消融术后房颤复发的保护因素(均P<0.05),心率[OR(95%CI):2.143(1.265~3.631)]、LAD[OR(95%CI):1.756(1.159~2.661)]为持续性心房颤动患者射频消融术后房颤复发的独立危险因素(均P<0.05)。miR-133a-3p,miR-324-3p二者联合预测持续性心房颤动患者射频消融术后房颤复发的AUC为0.901,敏感度和特异度分别为82.81%,86.21%,优于各自单独预测(Z=4.210,2.804,均P<0.05)。结论持续性心房颤动患者血清miR-133a-3p,miR-324-3p表达显著降低,二者联合对预测持续性心房颤动患者射频消融术后房颤复发有较好的参考价值。Objective To investigate the expression of serum miR-133a-3p and miR-324-3p in patients with persistent atrialfibrillation and their relationship with recurrence of atrialfibrillation after radiofrequency ablation.Methods A total of 180 patients with persistent atrialfibrillation(persistent atrialfibrillation group)who were hospitalized in Cangzhou City People’s Hospital from July 2019 to July 2022 and received radiofrequency ablation were collected as research objects.According to whether atrial fibrillation recurred,they were assigned into a non recurrence group(n=116)and a recurrence group(n=64).Meanwhile,another 180 healthy individuals who underwent physical examination at the hospital were regarded as the control group.The expression levels of serum miR-133a-3p and miR-324-3p of each group were compared.Multivariate Logistic regression was applied to analyze the factors influencing the recurrence of persistent atrial fibrillation after radiofrequency ablation,and ROC curve analysis was applied to analyze the predictive value of serum miR-133a-3p and miR-324-3p levels for the recurrence of atrialfibrillation in patients with persistent atrialfibrillation after radiofrequency ablation.Results Compared with the control group,the levels of serum miR-133a-3p(0.76±0.25)and miR-324-3p(0.68±0.21)in the persistent atrialfibrillation group were lower than the control group(1.03±0.32,1.05±0.30),and the differences were statistically significant(t=8.921,13.556,all P<0.05).The serum levels of miR-133a-3p(0.58±0.19)and miR-324-3p(0.50±0.16)in the recurrent group were obviously lower than those in the non recurrent group(0.86±0.27,0.78±0.25),and the differences were statistically significant(t=7.349,8.087,all P<0.05).Multivariate Logistic regression analysis showed that serum miR-133a-3p[OR(95%CI):0.673(0.534~0.848)]and miR-324-3p[OR(95%CI):0.756(0.629~0.909)]were protective factors for atrialfibrillation recurrence after radiofrequency ablation in patients with persistent atrialfibrillation,while heart rate[OR(9

关 键 词：持续性心房颤动 微小RNA-133a-3p 微小RNA-324-3p 射频消融术 房颤复发 

分 类 号：R541.75[医药卫生—心血管疾病] R392.11[医药卫生—内科学]