二级生物安全实验室实验操作人员培训体系的构建  

Construction of a training system for laboratory operators in biosafety level 2 laboratories

作　　者：张倩倩 李丽 赵俊[2] 刘万里[2] ZHANG Qian-qian;LI Li;ZHAO Jun;LIU Wan-li(School of Public Health,Xinjiang Medical University,Urumqi,Xinjiang 830054,China;不详)

机构地区：[1]新疆医科大学公共卫生学院,新疆乌鲁木齐830054 [2]新疆维吾尔自治区疾病预防控制中心实验室管理办公室,新疆乌鲁木齐830002

出　　处：《现代预防医学》2024年第21期4027-4032,共6页Modern Preventive Medicine

基　　金：新疆维吾尔自治区卫生健康青年医学科技人才专项科研项目(WJWY-202348)。

摘　　要：目的 构建二级生物安全实验室实验操作人员培训体系,为提升人员知识水平,规范操作技能,降低实验室意外事件提供保障。方法 采用德尔菲法对分别来自北京、上海、新疆、福建、安徽、重庆、广州、深圳地区的17名生物安全领域专家进行两轮函询,确定指标体系,应用层次分析法确定指标权重。结果 两轮问卷回收率为94.44%、100.00%,专家权威系数为0.962、0.944,肯德尔协调系数(W)的值较高(均P<0.01),最终确定培训体系包括5个一级指标、21个二级指标、83个三级指标。结论 该培训体系结构完整、内容全面,有较强的科学性及可靠性,能为二级生物安全实验室实验操作人员培训及考核提供参考依据。Objective To establish a training system for laboratory operators in Biosafety Level 2 laboratories,aimed at enhancing the knowledge level of personnel,standardizing operational skills,and reducing laboratory accidents.Methods The Delphi method was employed to conduct two rounds of consultations with 17 experts in the field of biosafety from Beijing,Shanghai,Xinjiang,Fujian,Anhui,Chongqing,Guangzhou,and Shenzhen to determine the indicator system,while the Analytic Hierarchy Process(AHP) was used to establish the weights of the indicators.Results The response rates for the two rounds of questionnaires were 94.44% and 100.00%,respectively,with expert authority coefficients of 0.962 and 0.944.The Kendall's coefficient of concordance(W) was high(all P < 0.01).Ultimately,the training system was defined to include 5 primary indicators,21 secondary indicators,and 83 tertiary indicators.Conclusion This training system is structurally complete and content-rich,demonstrating strong scientific validity and reliability,and can serve as a reference for the training and as-sessment of laboratory operators in Biosafety Level 2 laboratories.

关 键 词：二级生物安全实验室 实验操作人员 培训体系 权重 德尔菲法 

分 类 号：C975[经济管理—劳动经济]