甲醇胁迫培养下稀土元素镧对人体肠道微生物群落动态的影响  

Effect of the rare earth element lanthanum on community dynamics of methanol-grown fecal microbiota

作　　者：王菁妍 翟星宇 陈浩 彭璇 赵一鸣 肖颖 黄静 余正 WANG JingYan;ZHAI XingYu;CHEN Hao;PENG Xuan;ZHAO YiMing;XIAO Ying;HUANG Jing;YU Zheng(Human Microbiome and Health Group,Department of Microbiology,School of Basic Medical Science,Central South University,Changsha 410013,China;Department of Parasitology,School of Basic Medical Science,Central South University,Changsha 410013,China)

机构地区：[1]人体微生物与健康组,中南大学基础医学院医学微生物学系,长沙410013 [2]中南大学基础医学院医学寄生虫学系,长沙410013

出　　处：《中国科学：生命科学》2024年第11期2208-2219,共12页Scientia Sinica(Vitae)

基　　金：国家自然科学基金(批准号:32170071,32300051)资助。

摘　　要：稀土元素镧(Lanthanum,La^(3+))在工业、农业和生物医学等领域应用广泛.研究表明,La^(3+)在细菌甲基营养代谢调节中起着关键作用,但其在人类肠道微生物组中扮演的角色知之甚少.通过结合培养组学和高通量测序的方法,本研究揭示了添加La^(3+)后人体肠道菌群的反应动力学.我们以甲醇为唯一碳源的强选择性硝酸盐培养基(nitrate minimal salts,NMS)对收集到的肠道菌群样品进行培养,并测定了La^(3+)添加组(以下简称La组)与对照组(control,以下简称Con组)的群落组成变化.研究结果显示,经过培养后,甲基营养菌嗜甲基菌科(Methylophilaceae)是科水平上的优势菌,而嗜甲基菌属(Methylophilus sp.)是属水平上相对丰度更高的类群.此外,与对照组相比,添加La^(3+)后降低了培养样本中细菌种群的物种多样性,并引起了样品中物种组成的显著变化.值得注意的是,La^(3+)的加入还促进了嗜甲基菌属(Methylophilus sp.)与其他属之间的相互作用关系.本研究证实了甲基营养菌在肠道微生态系统结构和功能维持中的重要性,同时为揭示肠道菌群参与人体碳代谢机制研究提供了新的思路.Rare earth element lanthanum(La^(3+))is widely used in industry,agriculture and biomedicine.Previous studies have shown that La^(3+)plays a key role in methylotrophic regulation,but little is known about its role in human gut microbiome.Here,we reported the dynamics of bacterial communities in response to La^(3+)stimulation in human fecal samples by combining culturomics and highthroughput sequencing.Bacterial samples were cultured in strongly selective medium nitrate minimal salts(NMS)medium with methanol as the sole carbon source.Through measurements of the community-composition of enriched cultures under different treatments,we found that,Methylophilaceae family were dominant in the fecal culture samples.Furthermore,the Methylophilus was identified as the predominant genus.Additionally,the species diversity in the La^(3+)-added group was significantly reduced and the composition of species in cultured samples altered as well.Meanwhile,the addition of La^(3+)could promote the degree of connectivity between Methylophilus and other genera.This study not only affirms the crucial role of methylotrophic bacteria in preserving the structure and function of the intestinal microecosystem but also introduces innovative avenues for exploring methods and mechanisms that elucidate the participation of gut bacteria in single carbon metabolism of the human body.

关 键 词：镧元素 肠道菌群动态 嗜甲基菌科(Methylophilaceae) 培养组学 高通量测序 

分 类 号：R37[医药卫生—病原生物学]