电化学发光化学与生物传感方法  

Electrochemiluminescence chemical and biosensing methods

作　　者：漆红兰[1] 李延 张成孝[1] Honglan Qi;Yan Li;Chengxiao Zhang(Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province,School of Chemistry and Chemical Engineering,Shaanxi Normal University,Xi’an 710062,China;Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of the Ministry of Education,College of Chemistry and Materials Science,Northwest University,Xi’an 710069,China)

机构地区：[1]陕西省生命分析化学重点实验室,陕西师范大学化学化工学院,西安710062 [2]合成与天然功能分子化学教育部重点实验室,西北大学化学与材料科学学院,西安710069

出　　处：《中国科学：化学》2024年第10期1670-1688,共19页SCIENTIA SINICA Chimica

基　　金：国家自然科学基金(编号:22274093,22374094);中央高校基础研究经费项目(编号:GK202202002)资助项目。

摘　　要：本文重点介绍了本实验室在电化学发光(ECL)化学与生物传感方法方面的研究工作,简要介绍了ECL化学传感方法(流动注射法、能量转移法和偶合化学反应法),重点介绍了ECL生物传感方法(均相免疫、DNA杂交、适配体、凝集素、DNA酶、寡肽等)测定生物标志物的基本原理(包括ECL信号探针的设计、生物传感界面构筑和信号放大策略等),最后探讨了ECL化学与生物传感方法的挑战和发展前景.This Feature article mainly presents the research work of the author's laboratory in the field of chemical and biosensing methods based on electrochemiluminescence(ECL).This article extensively describes the basic principles of not only ECL chemical sensing methods(flow injection,energy transfer and chemical coupling) but also ECL biosensing methods(homogeneous immunoassay,DNA hybridization,aptamer,lectin,DNAzyme,oligopeptide,etc.)for the determination of biomarkers,highlights the design of ECL signal probes,construction of biosensing interfaces,and signal amplification strategies with illustrative examples,and presents the challenges and perspectives of ECL chemical and biosensing methods.

关 键 词：电化学发光 生物传感 生物标志物 分子识别物质 

分 类 号：O657.1[理学—分析化学] TP212.3[理学—化学] Q503[自动化与计算机技术—检测技术与自动化装置]