流式微球技术在生命分析化学领域的研究进展  

Research progress of flow cytometric bead technology in bioanalytical chemistry

作　　者：范文娇 任伟 董园园 刘成辉 Wenjiao Fan;Wei Ren;Yuanyuan Dong;Chenghui Liu(School of Chemistry&Chemical Engineering,Key Laboratory of Applied Surface and Colloid Chemistry(Ministry of Education),Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province,Shaanxi Normal University,Xi’an 710119,China)

机构地区：[1]陕西师范大学化学化工学院,应用表面与胶体化学教育部重点实验室,陕西省生命分析化学重点实验室,西安710119

出　　处：《中国科学：化学》2024年第10期1800-1816,共17页SCIENTIA SINICA Chimica

基　　金：国家自然科学基金(编号:22304110,22074088,21622507)资助项目。

摘　　要：流式微球检测技术以微米级颗粒为独立检测单元,以流式细胞仪为信号读出手段,具有高灵敏度、高通量、多维度信息读取、快速检测等优势,并且可以与各种信号放大/扩增技术以及编码技术结合,实现对多种生物标志物的高灵敏度检测,在分析化学领域得到了广泛应用.此外,数字式流式单分子检测技术和生物微颗粒载体流式传感技术也在提升流式检测性能、扩展流式检测应用范围等方面展现出了独特的魅力.本文概述了流式微球检测技术在生命分析化学领域的研究进展,系统归纳了各类流式微球检测方法的特点,并指出了该技术面临的挑战与未来发展前景.Flow cytometric bead technology employs microparticles as individual detection units and flow cytometer as signal readout instrument,is endowed with the advantages of high sensitivity,high throughput,and multi-dimensional information acquisition and rapid detection capabilities.It can be combined with various signal amplification and encoding strategies to realize highly sensitive multiplexed biomarker analysis,which has been widely used in the field of analytical chemistry.More fascinatingly,emerging single-molecule level digital flow cytometric strategies as well as the biological microparticles-based flow cytometric biosensors can significantly improve the detection performance and expand the application scenario of the flow cytometric technology.In this paper,the research progress of flow cytometric bead technology in the field of bioanalytical chemistry is reviewed,the characteristics of various kinds of flow cytometric bead methods are systematically summarized,and the challenges and future prospects of this technology are also discussed.

关 键 词：流式微球 信号扩增 多重检测 数字式分析 生物微颗粒 

分 类 号：Q503[生物学—生物化学]