“非电极”限域电化学发光成像免疫分析新方法检测甲胎蛋白  

Electrogenerated chemiluminescence imaging immunoassay of alpha fetoprotein at non-electrode confined surface

作　　者：李钊 冯岩龙 周文帅 杨小林 张成孝[1] 漆红兰[1] Zhao Li;Yanlong Feng;Wenshuai Zhou;Xiaolin Yang;Chengxiao Zhang;Honglan Qi(Key Laboratory of Applied Surface and Colloid Chemistry of Ministry of Education,Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province,School of Chemistry and Chemical Engineering,Shaanxi Normal University,Xi’an 710062,China)

机构地区：[1]应用表面与胶体化学教育部重点实验室,陕西省生命分析化学重点实验室,陕西师范大学化学化工学院,西安710062

出　　处：《中国科学：化学》2024年第10期1935-1942,共8页SCIENTIA SINICA Chimica

基　　金：国家自然科学基金(编号:22374094);中央高校基础研究经费项目(编号:GK202202002);陕西师范大学研究生领航人才培养项目(编号:LHRCTS23073)资助项目。

摘　　要：本文提出一种非电极限域电化学发光(ECL)成像免疫分析新策略,即负载信号物质的磁性微粒(发光磁性微粒)分散在不导电的载玻片上,与裸玻碳电极组成双界面工作电极体系;在共反应试剂存在下,裸玻碳电极上施加外加电压产生的自由基与载玻片上限域的信号物质发生化学反应,产生ECL信号.结果发现,当双界面电极体系之间距离≤15μm时,可观察到发光磁性微粒的ECL信号;当其距离为18μm时,观察不到ECL信号;在5.0~12.5μm之间增加磁性微粒尺寸,ECL强度逐渐减小.以5.0μm磁性微粒为捕获载体,探索建立了检测甲胎蛋白(AFP)的“非电极”限域电化学发光成像免疫分析新方法,AFP浓度在10~200 ng·m L^(-1)范围内与ECL强度呈正比,检出限为2.2 ng·m L^(-1).该研究工作为电化学发光微米颗粒成像、生物传感分析法研究提供了新思路.A new non-electrode confined electrogenerated chemiluminescence (ECL) imaging immunoassay was proposed,in which non-conductive glass slide modified with signal reagent modified magnetic particles (as luminescent MB) was combined with bare glassy carbon electrode to form a dual interface working electrode system.In the presence of co-reactant reagents,the free radicals electrogenerated at bare glassy carbon electrode reacted with the signal substance on non-conductive glass slide,producing ECL emission.It was found that obvious ECL signal can be observed from the luminescent MBs when the distance between the dual interface electrode systems was≤15μm while no ECL signal can be observed when the distance is 18μm;The ECL intensity gradually decreased with the increase of MB size between 5.0–12.5μm.A new“non-electrode”confined ECL imaging immunoassay for alpha fetoprotein was developed using 5.0μm MB with a detection limit of 2.2 ng·m L^(-1).This work provides a promising idea and method for ECL imaging of microparticle and biosensing analysis.

关 键 词：电化学发光 成像分析 微米颗粒 免疫分析 蛋白质 

分 类 号：O657.1[理学—分析化学] R446.1[理学—化学]