蒸脐疗法治疗脾虚湿盛型腹泻型肠易激综合征模型大鼠的机制研究  

echanism study of steaming navel therapy in the treatment of spleen deficiency and dampness type diarrhea-predominant irritable bowel syndrome model rats

作　　者：廖正仪 葛来安[2] LIAO Zhengyi;GE Laian(Jiangxi University of Traditional Chinese Medicine,Nanchang,330004,China;Department of Gastroenterology,Jiangxi Provincial Hospital of Traditional Chinese Medicine)

机构地区：[1]江西中医药大学,南昌330004 [2]江西省中医院消化一科

出　　处：《中国中西医结合消化杂志》2024年第11期979-984,共6页Chinese Journal of Integrated Traditional and Western Medicine on Digestion

基　　金：国家自然科学基金项目(No:82060840)。

摘　　要：目的研究蒸脐疗法治疗脾虚湿盛型腹泻型肠易激综合征(diarrhea-predominant irritable bowel syndrome,IBS-D)模型大鼠的作用机制。方法将40只SD雄性大鼠分为空白组、模型组、中药蒸脐组、西药对照组,每组各10只。采用番泻叶浸出液灌胃给药联合慢性温和束缚应激加孤养法建立大鼠IBS-D模型。造模成功后,各组采用相应的处理方法连续干预14 d,观察大鼠一般情况、体重、粪便干湿比重及腹部回缩反射(abdominal wall retraction,AWR)评分。用ELISA法测定大鼠血清、结肠、海马组织中P物质(substance P,SP)的含量,对结肠组织进行病理学观察。结果①蒸脐疗法可显著改善IBS-D模型大鼠的精神状态、排便情况、粪便性状、被毛颜色等一般情况(P<0.05)并恢复其体重的正常增长(P<0.05);②蒸脐疗法可降低大鼠海马、血清、结肠组织中SP的含量(P<0.01)及AWR评分(P<0.01),调控内脏高敏性,减轻肠道炎症,从而缓解IBS-D的临床症状。结论蒸脐疗法可能是通过调控SP水平,降低内脏高敏性,从而达到治疗脾虚湿盛型IBS-D大鼠的目的。Objective To investigate the mechanism of steaming navel therapy in treating diarrhea-predominant irritable bowel syndrome(IBS-D)model rats with spleen deficiency and dampness.Methods Forty male SD rats were divided into a blank group,a model group,a traditional Chinese medicine navel steaming group,and a western medicine control group,with 10 rats in each group.A rat model of IBS-D was established by gavage administration of senna leaf extract combined with chronic mild restraint stress and solitary confinement.After successful modeling,each group was intervened continuously for 14 days using corresponding treatment methods,and the general condition,body weight,fecal dry wet density,and abdominal wall retraction(AWR)score of the rats were observed.The content of substance P(SP)in rat serum,colon,and hippocampus tissue was measured using the ELISA method,and perform pathological observation on colon tissue.Results①Umbilical steaming therapy can significantly improve the general conditions such as mental state,defecation,fecal characteristics,and coat color of IBS-D model rats(P<0.05)and restore normal weight growth(P<0.05);②Umbilical steaming therapy can reduce the content of SP in hippocampus,serum,and colon tissues(P<0.01),lower the AWR score of rats(P<0.01),regulate visceral hypersensitivity,reduce intestinal inflammation,and alleviate clinical symptoms of IBS-D.Conclusion Steaming navel therapy may achieve the goal of treating spleen deficiency and dampness excess type of IBS-D by regulating substance P and reducing visceral hypersensitivity.

关 键 词：腹泻型肠易激综合征 蒸脐疗法 P物质 内脏高敏性 脾虚湿盛 

分 类 号：R259[医药卫生—中西医结合]