RhD变异体Type33型受血者同种免疫产生抗-D合并抗-E混合抗体的研究  

Studyon the Production of Anti-D and Anti-E Mixed Antibodies by Alloimmunizationin RhD Variant Type33 Recipients

作　　者：刘建成 邵峰 LIU Jian-Cheng;SHAO Feng(Blood Group Reference Laboratory,Ningxia Blood Center,Yinchuan 750000,Ningxia Hui Autonomous Region,China)

机构地区：[1]宁夏血液中心血型参比实验室,宁夏银川750000

出　　处：《中国实验血液学杂志》2024年第6期1859-1864,共6页Journal of Experimental Hematology

基　　金：宁夏卫生健康系统科研课题(2022-NWKY-040)。

摘　　要：目的:探讨1例RhD阳性患者免疫产生抗-D合并抗-E混合抗体的原因。方法:用常规血清学方法鉴定ABO/Rh血型和不规则抗体特异性,采用序列特异性引物聚合酶链式反应(PCR-SSP)检测患者的RHD基因外显子1-10区域并进行杂合性分析,利用一代测序方法分析全外显子序列。结果:患者Rh血型为弱D Type33型,其RHD等位基因为RHD01W.33,患者表现为RHD+/RHD-杂合型,Rh分型为Ccee,产生了抗-D合并抗-E混合抗体。结论:该患者RHD基因外显子4发生了c.520G>A的突变,这一变异导致了红细胞上RhD抗原的表达减弱。Objective:To investigate the cause of the production of anti-D and anti-E mixed antibody in an RhD positive patient.Methods:The ABO/Rh blood group typing and irregular antibody specificity were identified by conventional serological methods,the RHD gene exon 1-10 and heterozygous analysis were performed by sequence-specific primer polymerase chain reaction(PCR-SSP),and the whole exon sequence was analyzed by first-generation sequencing.Results:The patient’s Rh blood group was weak D Type33,with the allele was RHD01W.33,the patients was found to be RhD+/RHD-heterozygous,with an Rh typing of Ccee,and the patient had developed anti-D combined with anti-E mixed antibodies.Conclusion:The patient has A c.520G>A mutation in exon 4 of the RHD gene,to lead decreased expression of RhD antigen in red blood cells and the anti-D and anti-E mixed antibodies were produced by transfusion immunostimulation.

关 键 词：弱D Type33型 RhD变异体 Rh血型 RHD基因 混合抗体 受血者 

分 类 号：R457.1[医药卫生—治疗学]