重组人钙网蛋白促雄激素性脱发小鼠毛发再生的作用研究  

Recombinant human calreticulin promotes hair regeneration in androgenetic alopecia mice

作　　者：贺改英 吴首标 刘树涛 魏东东 崔俊生 王毅[1] Gaiying He;Shoubiao Wu;Shutao Liu;Dongdong Wei;Junsheng Cui;Yi Wang(Experimental Research Center,China Academy of Chinese Medical Sciences,Beijing 100700,China;Beijing BioHelix Biotech Co.,Ltd.,Beijing 102308,China)

机构地区：[1]中国中医科学院医学实验中心,北京100700 [2]北京百华百汇生物科技有限公司,北京102308

出　　处：《日用化学工业（中英文）》2024年第11期1313-1319,共7页China Surfactant Detergent & Cosmetics

基　　金：国家自然科学基金青年基金(82204902);中央级公益性科研院所基本科研业务费(JBGS2023003)。

摘　　要：研究重组人钙网蛋白(recombinant human calreticulin,rh-CRT)促雄激素性脱发(androgenetic alopecia,AGA)小鼠毛发再生的作用。建立AGA小鼠模型,局部应用rh-CRT,研究rh-CRT对毛发再生的影响。通过观察不同时间毛发整体外观和局部外观评估毛发的再生,采用颜色测试仪检测皮肤的黑色素指数,利用皮肤镜观察不同类型毛发直径的变化,扫描电镜检测毛小皮的改变,并进一步通过HE染色研究皮肤和真皮脂肪厚度以及毛囊的数量。结果发现,rh-CRT促进AGA小鼠毛发的再生,增加黑色素指数,并显著提高AGA小鼠粗毛和细毛的直径,修复睾酮组(testosterone,TES)诱导的毛小皮的损伤。从皮肤冠状面观察发现rh-CRT显著促进AGA小鼠毛囊数量的表达,并增加皮肤和真皮脂肪的厚度。这些结果表明rh-CRT可以促进AGA小鼠毛发的再生,在防脱产品中具有重要的应用价值。Androgenetic alopecia(AGA)is the most common type of hair loss and is usually caused by multiple factors.AGA is characterized by progressive miniaturization of hair follicles,shortened anagen phase,prolonged telogen phase,and the decrease of hair density.We studied the role of recombinant human calreticulin(rh-CRT)in promoting hair regeneration in androgenetic alopecia(AGA)mice.Hair regeneration was assessed by observing the overall and local appearance of the hair at different time points,the melanin index of the skin was observed by color tester,the change of hair diameter was detected by dermatoscopy,the change of the hair cuticle was studied by scanning electron microscopy,and the thickness of the skin and the dermal white adipose,and the number of hair follicles were further observed by HE staining.The results reveal that rh-CRT promotes hair regeneration,increases melanin index,significantly improves the diameters of coarse and fine hairs in AGA mice,and repairs the damaged hair cuticle induced by testosterone(TES).In addition,it is demonstrated that rh-CRT significantly promotes the number of hair follicles and the thickness of the skin and dermal white adipose in AGA mice.The results suggest that rh-CRT promotes the regeneration of hair in AGA mice,which has important application value in hair regeneration products.

关 键 词：重组人钙网蛋白 雄激素性脱发 毛发再生 

分 类 号：TQ658[化学工程—精细化工]