异落新妇苷的美白作用及抗氧化作用研究  

Study on the whitening effect and antioxidation of isoastilbin

作　　者：刘慧 刘芳 Hui Liu;Fang Liu(Xinyang Vocational and Technical College,Xinyang,Henan 464000,China;People’s Hospital of Quenshan,Zhumadian,Henan 463299,China)

机构地区：[1]信阳职业技术学院,河南信阳464000 [2]确山县人民医院,河南驻马店463299

出　　处：《日用化学工业（中英文）》2024年第11期1368-1374,共7页China Surfactant Detergent & Cosmetics

摘　　要：为了揭示异落新妇苷(isoastilbin,ISO)的美白作用及抗氧化作用。建立了紫外线B(UVB)诱导的皮肤光老化(SP)小鼠模型,使用3个剂量(1,2,4 mg/cm^(2))的异落新妇苷对小鼠进行经皮给药。治疗8周后,检测了小鼠的表皮含水量。通过HE染色和Masson三色染色观察皮肤表皮和真皮形态。按试剂盒说明书检测皮肤氧化应激指标(超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)和丙二醛(MDA))水平。采用NaOH溶解法检测皮肤黑色素含量。采用酶联免疫吸附剂测定(ELISA)法检测皮肤酪氨酸酶(TYR)活性。通过RT-qPCT检测皮肤基质金属蛋白酶-1(MMP-1)和CollagenⅠ的mRNA水平。通过Western blotting检测皮肤细胞核核因子红细胞2相关因子2(Nrf2)的蛋白表达水平。结果显示,异落新妇苷剂量依赖性地提高了UVB诱导的SP小鼠的表皮含水量,降低了表皮厚度,减少了胶原纤维变性,降低了MMP-1的mRNA相对表达量,升高了CollagenⅠ的mRNA相对表达量,升高了SOD、CAT和GSH-Px活性,降低了MDA含量,升高了细胞核Nrf2的蛋白相对表达量,降低了黑色素含量和TYR活性(P<0.05)。本研究表明,异落新妇苷可减轻UVB诱导的小鼠SP,并具有美白作用,其机制与提高皮肤抗氧化能力以及降低黑色素含量和TYR活性有关。The study aimed to reveal the whitening effect and antioxidant effect of isoastilbin(ISO).In this study,a mouse model of skin photoaging(SP)induced by ultraviolet B(UVB)was established.Three doses(1,2,4 mg/cm^(2))of isoastilbin were administered transcutaneously in mice.After 8 weeks of treatment,the water content of mouse epidermis was detected.The morphology of epidermis and dermis was observed by HE staining and Masson trichrome staining.The levels of skin oxidative stress indicators(superoxide dismutase(SOD),catalase(CAT),glutathione peroxidase(GSH-Px)and malondialdehyde(MDA))were measured according to the instructions of the kit.The content of melanin in skin was detected by NaOH lysis method.The activity of tyrosinase(TYR)in the skin was detected by enzyme-linked immunosorbent assay(ELISA).The mRNA levels of matrix metalloproteinase-1(MMP-1)and Collagen I were detected by RT-qPCT.The protein expression of nuclear factor E2-related factor 2(Nrf2)in the skin cells was detected by Western blotting.The results show that isoastilbin increases the water content of epidermis,decreases the thickness of epidermis,reduces the degeneration of collagen fibers,decreases the relative expression of mRNA of MMP-1,increases the relative expression of mRNA of Collagen I,increases the activities of SOD,CAT and GSH-Px,decreases the content of MDA,increases the relative expression of nuclear Nrf2 and decreases the content of melanin and TYR in SP mice induced by UVB in a dose-dependent manner(P<0.05).This study shows that isoastilbin can reduce UVB-induced SP in mice and has a whitening effect,which is related to the improvement of skin antioxidant capacity and the decrease of melanin content and TYR activity.

关 键 词：异落新妇苷 紫外线B 美白 抗氧化 皮肤光老化 黑色素 酪氨酸酶 

分 类 号：TQ658[化学工程—精细化工]