1例致滇金丝猴肠炎大肠杆菌的分离鉴定及耐药性分析  

Isolation,identification and drug resistance analysis of a case of Escherichia coli causing enteritidis in Yunnan snub-nosed monkey

作　　者：陈雅静 于婧 杨劲宇 张文公 吴玉 刘凇昊 杨静 李小兵 江康峰 CHEN Yajing;YU Jing;YANG Jinyu;ZHANG Wengong;WU Yu;LIU Songhao;YANG Jing;LI Xiaobing;JIANG Kangfeng(College of Veterinary Medicine,Yunnan Agricultural University,Kunming 65020l,China;Animal Husbandry Workstation of Jianchuan County,Dali,Yunnan 671000,China)

机构地区：[1]云南农业大学动物医学院,云南昆明650201 [2]云南省剑川县畜牧工作站,云南大理671000

出　　处：《中国兽医学报》2024年第10期2130-2135,2265,共7页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金青年科学基金资助项目(32202885);云南省基础研究专项资助项目(202301AT070492)。

摘　　要：通过将昆明市某动物园1例因呕吐、口鼻流食糜、腹部鼓胀死亡滇金丝猴的胃脏、空肠、直肠组织样品的病原进行分离纯化,利用生化鉴定、PCR鉴定、药敏试验、致病性试验、血清型鉴定及对分离菌进行耐药基因和毒力基因分析,对病原种类和生物学特性进行研究。结果显示,从胃脏、空肠和直肠中分离鉴定到的病原为大肠杆菌,血清型均为O127,属于肠致病性大肠杆菌,且均对头孢西丁耐药,对庆大霉素、加替沙星和环丙沙星敏感。3株致病菌均携带blaTEM、blaCTX-M 2种耐药基因以及opmA、opmC 2种毒力基因。本试验结果为大肠杆菌致滇金丝猴肠炎的防治提供了一定的参考和数据支持。The pathogens were isolated and purified from the stomach,jejunum and rectum tissues of a Yunnan snub-nosed monkey who died of vomiting,oral and nasal chyme,and abdominal distension,and the species and biological characteristics of the pathogens were studied by biochemical identification,PCR identification,drug susceptibility test,pathogenicity test,serotype identification,and drug resistance gene and virulence gene analysis.The results showed that the pathogens isolated from stomach,jejunum and rectum were Escherichia coli(E.coli)serotype O127,belonging to enteropathogenic E.coli.They were resistant to cefoxitin and sensitive to gentamicin,gatifloxacin and ciprofloxacin.All the three strains carried drug resistance genes blaTEM and blaCTX-M and virulence genes opmA and opmC.This study provides reference and data support for the prevention and control of enteritis caused by E.coli in Yunnan snub-nosed monkey.

关 键 词：滇金丝猴 肠炎 致病性大肠杆菌 耐药基因 毒力基因 

分 类 号：S852.61[农业科学—基础兽医学]