板蓝根多糖通过TLR3/TRIF通路抑制PRRSV复制  

Radix isatidis polysaccharide suppresses PRRSV replication through the TLR3/TRIF pathway

作　　者：吴文义 胡雪岩 张云天 张志龙 李倩楠 金钺[1,2,3] 杨明凡 张红英[1,2,3] WU Wenyi;HU Xueyan;ZHANG Yuntian;ZHANG Zhilong;LI Qiannan;JIN Yue;YANG Mingfan;ZHANG Hongying(College of Veterinary Medicine,Henan Agriculture University,Zhengzhou 450002,China;Key Laboratory of Animal Food Safety in Henan Province,Zhengzhou 450002,China;Zhengzhou City Key Laboratory for Research and Evaluation of Chinese Veterinary Drugs,Zhengzhou 450002,China)

机构地区：[1]河南农业大学动物医学院,河南郑州450002 [2]河南省动物性食品安全重点实验室,河南郑州450002 [3]郑州市中兽药研究与评价重点实验室,河南郑州450002

出　　处：《中国兽医学报》2024年第10期2197-2203,共7页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(31972731)。

摘　　要：通过Western blot、ELISA方法检测板蓝根多糖(Radix isatidis polysaccharide,IRPS)对猪繁殖与呼吸综合征病毒(porcine reproductive and respiratory syndrome virus,PRRSV)感染的3D4/21/CD163细胞TLR3/TRIF天然免疫途径和Ⅰ型干扰素分泌的影响,并使用TLR3激动剂Poly(I:C)处理进一步验证IRPS对PRRSV感染引起的免疫抑制的影响。结果显示,PRRSV感染18、24 h时TLR3、TRIF、IRF3、IRF7蛋白水平及Ⅰ型干扰素分泌均显著下调,同时IRPS对这一过程具有显著的抑制作用;TLR3激动剂Poly(I:C)能够缓解PRRSV感染引起的TRIF、IRF3、IRF7蛋白水平下调和IRF3、IRF7磷酸化水平降低,且IRPS与Poly(I:C)具有协同作用。结果表明,IRPS能够通过TLR3/TRIF途径缓解PRRSV感染引起的免疫抑制。The effect of Radix isatidis polysaccharide(IRPS)on TLR3/TRIF innate immune pathway and typeⅠinterferon secretion in 3D4/21/CD163 cells infected by porcine reproductive and respiratory syndrome virus(PRRSV)was tested by Western blot and ELISA;moreover,the effect of IRPS on the immunosuppression infected by PRRSV was further verified with the TLR3 agonist poly(I:C).The results showed that the protein levels of TLR3,TRIF,IRF3,IRF7 and typeⅠinterferon secretion were significantly decreased at 18,24 h of PRRSV infection,while IRPS significantly inhibited this process;poly(I:C)alleviated the protein levels of TRIF,IRF3 and IRF7 as well as the phosphorylation levels of IRF3 and IRF7 infected by PRRSV;at the same time,IRPS is synergistic with poly(I:C).The results indicate that IRPS is able to alleviate immunosuppression caused by PRRSV infection via the TLR3/TRIF pathway.

关 键 词：IRPS PRRSV TLR3 Ⅰ型干扰素 

分 类 号：S852.2[农业科学—基础兽医学] S859[农业科学—兽医学]