木通与蒲公英提取物对断奶仔兔肠道氧化应激的改善作用  

Effects of akebiaquinata and dandelion extracts on intestinal oxidative stress in weaned rabbits

作　　者：张俊秋 陈雨 修子清 MUSA Mgeni 蒋小雨 吕景智[1] 孙雅望 ZHANG Junqiu;CHEN Yu;XIU Ziqing;MUSA Mgeni;JIANG Xiaoyu;LYU Jingzhi;SUN Yawang(College ofAnimal Scienceand Technology,Southwest University,Chongqing 400715,China;College of Veterinary Medicine,Southwest University,Chongqing 400715,China)

机构地区：[1]西南大学动物科学技术学院,重庆400715 [2]西南大学动物医学院,重庆400715

出　　处：《中国兽医学报》2024年第10期2221-2233,共13页Chinese Journal of Veterinary Science

摘　　要：选用35日龄、体质量(1.22±0.08)kg的伊拉兔120只,根据双因素设计随机地分为4组:C组(基础饲粮)、D组(基础饲粮+0.5%蒲公英提取物)、A组(基础饲粮+0.5%木通提取物)、DA组(基础饲粮+0.5%蒲公英提取物+0.5%木通提取物),其中,C组为对照组,其余3组为试验组,每组10个重复。预饲期1周,试验期4周。试验结束后采集血清、肝脏组织、空肠和回肠黏膜样本,并测定各项指标。结果显示:(1)在试验期第1周,C组仔兔的平均日增重显著低于D组和A组(P<0.05),料重比显著高于D组和A组(P<0.05)。(2)木通提取物极显著降低了肝脏和血清中的活性氧(reactive oxygen species,ROS)含量(P<0.01),蒲公英提取物极显著降低了血清ROS含量(P<0.01),且分别在肝脏和血清中存在显著和极显著交互作用;木通和蒲公英提取物能有效降低组织和血清中氧化损伤标志物含量,但增加了肝脏组织中丙二醛(malondialehyde,MDA)的含量。(3)木通提取物显著提高了血清中谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活性和总抗氧能力(total antioxidant capability,T-AOC)(P<0.01),并极显著降低了肝脏中的T-AOC(P<0.01),显著降低空肠和肝脏中超氧化物歧化酶的活性(P<0.05);蒲公英提取物显著提高血清中T-AOC和空肠中GSH-Px活性(P<0.05);木通和蒲公英提取物对血清中的过氧化物酶具有显著互作效应(P<0.05)。(4)木通提取物显著和极显著的降低了Kelch样结合蛋白1(Kelch-like ECH-associated protein 1,Keap1)和NAD(P)H醌氧化还原酶1(NAD(P)H:quinone oxidoreductase 1,NQO1)基因在空肠的表达量;木通和蒲公英提取物对回肠NQO1、血红素加氧酶1和超氧化物歧化酶2有显著互作效应(P<0.05),对Keap1有极显著互作效应(P<0.01);木通提取物显著降低NQO1基因在肝脏组织表达量(P<0.05)。结果表明,蒲公英和木通提取物能够减少体内ROS含量并缓解氧化损伤,并通过Nrf2-Keap1信号通路调节抗氧化基因表达和抗氧化�This study aimed to explore the effects of akebiaquinata and dandelion extracts in improving intestinal redox homeostasis in weaned rabbits.In the trial,12035-day-old Ira rabbits weighing(1.22±0.08)kg were randomly divided into 4 groups according to the two-factor design,namely group C(basal diet),group D(basal diet+0.5%dandelion extract),group A(basal diet+0.5%akebiaquinata extract),and group DA(basal diet+0.5%dandelion extract+0.5%akebiaquinata extract),with 10 replicates in each group.The adapt period was one week and the experimental period was four weeks.At the last day,serum,liver tissue,jejunum and ileal mucosa samples were collected and stored for measurement.The results showed that:(1)First week,the average daily weight gain of group C was significantly lower than that of group D and group A(P<0.05),and the feed weight ratio was significantly higher than that of group D and group A(P<0.05).(2)The content of reactive oxygen species(ROS)in liver and serum was significantly reduced in akebiaquinata extract(P<0.01),and the content of serum ROS in dandelion extract was significantly reduced(P<0.01),and there were significant and extremely significant interactions in liver and serum,respectively.Extracts of akebiaquinata and dandelion were effective in reducing the levels of oxidative damage markers in tissues and serum,but increasing the content of malondialdehyde in liver tissues.(3)Akebiaquinata extract significantly increased the activity of glutathione peroxidase(GSH-Px)and total antioxidant capacity(T-AOC)in serum(P<0.01),and significantly reduced the activity of T-AOC in liver(P<0.01)and superoxide dismutase in jejunum and liver(P<0.05).Dandelion extract significantly increased the activity of T-AOC in serum and GSH-Px in jejunum(P<0.05).The extracts of akebiaquinata and dandelion had a significant interaction effect on peroxidase in serum(P<0.05).(4)The expression of Kelch-like ECH-associated protein 1(Keap1)and NAD(P)H:quinone oxidoreductase 1(NQO1)genes in the jejunum was significantly and extremely

关 键 词：蒲公英 木通 断奶仔兔 生产性能 肠道氧化应激 

分 类 号：S852.2[农业科学—基础兽医学] S859[农业科学—兽医学]