电感耦合等离子体质谱技术检测单细胞内外源物质的研究进展  

Advances in Inductively Coupled Plasma-Mass Spectrometry for Detection of Endogenous and Exogenous Substances in Single Cells

作　　者：徐桃 田祥伟 刘艳伟 郭瑛瑛 胡立刚[1,2,3] 阴永光 张庆华[1,2,3] 蔡勇 江桂斌[1,2,3] XU Tao;TIAN Xiang-Wei;LIU Yan-Wei;GUO Ying-Ying;HU Li-Gang;YIN Yong-Guang;ZHANG Qing-Hua;CAI Yong;JIANG Gui-Bin(State Key Laboratory of Environmental Chemistry and Ecotoxicology,Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085,China;School of Environment,Hangzhou Institute for Advanced Study,University of Chinese Academy of Sciences,Hangzhou 310024,China;University of Chinese Academy of Sciences,Beijing 100049,China;Department of Chemistry and Biochemistry,Florida International University,Miami,FL 33199,United States of America)

机构地区：[1]中国科学院生态环境研究中心,环境化学与生态毒理学国家重点实验室,北京100085 [2]国科大杭州高等研究院环境学院,杭州310024 [3]中国科学院大学,北京100049 [4]美国佛罗里达国际大学化学与生物化学系,迈阿密33199

出　　处：《分析化学》2024年第10期1403-1412,共10页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(Nos.42394091,42277208)资助。

摘　　要：细胞是生物体的基本结构和功能单元,不同细胞的组成成分及其与外源物质的作用行为均存在差异(即细胞异质性)。单细胞电感耦合等离子体质谱技术(Single cell-inductively coupled plasma-mass spectrometry,SC-ICP-MS)通过高通量逐个引入单细胞,可对单个细胞中的元素进行高灵敏检测和定量分析,有效提供细胞异质性信息。本文介绍了不同类型细胞(单细胞体系、易团聚和贴壁细胞体系、动物组织和植物组织)的SC-ICP-MS单细胞样品制备流程,包括分离、洗涤和固定等步骤,以及目前通用的样品引入系统和定量方法的优势和存在的问题,总结了近年来SC-ICP-MS在检测细胞内源物质(内源元素和内源蛋白质)、外源物质(重金属、金属药物和纳米颗粒)和同时检测内外源物质等方面的应用,并对SC-ICP-MS在分析方法和应用领域方面的未来发展进行了展望,包括单细胞样品制备、传输效率和离子化效率评估标准的优化以及多参数细胞分析平台的建立等。Cells are the fundamental structural and functional units of biological organisms,with inherent differences in composition and interactions with exogenous substances,known as cellular heterogeneity.Single cell inductively coupled plasma-mass spectrometry(SC-ICP-MS)allows for the high-throughput introduction of individual cells,enabling the highly sensitive detection and quantification of elements within a single cell,thus effectively providing information on cellular heterogeneity.This review outlined the SC-ICP-MS sample preparation process for different types of cells(single-cell systems,aggregation-prone and adherent cell systems,animal tissues,and plant tissues),including steps such as separation,washing,and fixation,as well as the advantages and existing issues of the current sample introduction systems and quantification methods.The recent applications of SC-ICP-MS in detecting endogenous substances(endogenous elements and proteins),exogenous substances(heavy metals,metal-based drugs and nanoparticles),and the simultaneous detection of both endogenous and exogenous substances were summarized.Finally,the perspectives on the future development of SC-ICP-MS in analytical methods and application fields were presented,including the optimization of single-cell sample preparation,transport efficiency,evaluation standards of ionization efficiency,and the establishment of multiparametric cell analysis platforms.

关 键 词：电感耦合等离子体质谱 单细胞分析 金属 外源物质 内源物质 评述 

分 类 号：O657.63[理学—分析化学] Q2-33[理学—化学]