单颗粒电感耦合等离子体质谱技术用于生物基质中纳米银检测的研究进展  

Advances in Single Particle Inductively Coupled Plasma-Mass Spectrometry Analysis of Silver Nanoparticles in Biological Matrices

作　　者：邢国慧 刘丽红[1] 张俊辉 何滨[1,2] 阴永光 胡立刚[1,2] 江桂斌 XING Guo-Hui;LIU Li-Hong;ZHANG Jun-Hui;HE Bin;YIN Yong-Guang;HU Li-Gang;JIANG Gui-Bin(State Key Laboratory of Environmental Chemistry and Ecotoxicology,Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085,China;University of Chinse Academy of Sciences,Beijing 100085,China)

机构地区：[1]中国科学院生态环境研究中心,环境化学与生态毒理学国家重点实验室,北京100085 [2]中国科学院大学,北京100085

出　　处：《分析化学》2024年第10期1413-1423,共11页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(Nos.22193052,22076200,22276201);中国科学院青年促进会项目(No.2023048);中国科学院战略性先导科技专项(B类)项目(No.XDB0750200)资助。

摘　　要：纳米银(AgNPs)被广泛应用于生物医学、日化用品和食品工业等领域,其暴露引起的潜在健康影响引起了公众的广泛关注。生物基质中AgNPs的准确分析是研究AgNPs生物安全性的基础。在现有的分析检测技术中,单颗粒电感耦合等离子体质谱法(Single particle-inductively coupled plasma-mass spectrometry,sp-ICP-MS)具有分析速度快、灵敏度高、操作简单、可同时检测AgNPs与银离子形态等优势。然而,生物基质中的AgNPs具有形态动态变化、基质干扰严重等特点,对其前处理与分析有较高挑战性。目前已有一些关于sp-ICP-MS方法应用于不同场景中金属纳米颗粒检测的综述,但对生物基质中AgNPs的分析仍缺乏参考标准,难以实现准确的定量分析,因此需要对现有方法进行总结和进一步优化。本文从方法应用的角度回顾了近年来基于sp-ICP-MS技术分析生物基质中AgNPs的研究进展,包括颗粒提取方法、技术原理以及数据处理等方面,并探讨了目前sp-ICP-MS应用于生物组织中AgNPs检测存在的问题以及未来的发展趋势。Silver nanoparticles(AgNPs)is widely used in biomedicine,daily chemicals,food industry and other fields,and the possible negative health effects of its exposure have attracted widespread attention.Accurate analysis of AgNPs in biological matrices is the basis for biosafety studies of AgNPs.Among the existing analytical techniques,single particle-inductively coupled plasma-mass spectrometry(sp-ICP-MS)has significant advantages such as high sensitivity and simultaneous detection of different forms of silver.However,AgNPs in biological matrices is uniquely highly dynamic and low in content,and the matrix interference is severe,which increases the complexity of the analysis.Although some scholars have reviewed the application of this method for detection of metal nanoparticles in different scenarios,there is a lack of a summary of the quality control and optimization of the whole process from the perspective of AgNPs detection.There is still a lack of reference standards for the sp-ICPMS analysis of AgNPs in biological matrices,and the existing methods need to be summarized and further optimized to achieve accurate quantification.Therefore,this paper reviewed the recent studies on the analysis of silver-containing nanoparticles in biological matrices based on sp-ICP-MS,mainly included the principles of the technique,the extraction methods of the particles,and the process of data processing,which focused on elaborating and comparing different pre-treatment methods,and explored issues of the current application of sp-ICP-MS for detection of AgNPs in biological tissues and the development of future optimization trends.The current problems of sp-ICP-MS for detection of AgNPs in biological tissues and the future development trend were also discussed.

关 键 词：单颗粒电感耦合等离子体质谱法 纳米银 生物基质 前处理技术 评述 

分 类 号：O657.63[理学—分析化学]