水解纳米酶的类酶催化及传感应用研究进展  

Recent Advances in Nanozymes with Hydrolase-like Activity for Sensing Application

作　　者：姜文轩 吴俣 许维庆 周莹 朱成周 林跃河 JIANG Wen-Xuan;WU Yu;XU Wei-Qing;ZHOU Ying;ZHU Cheng-Zhou;LIN Yue-He(International Joint Research Center for Intelligent Biosensing Technology and Health,State Key Laboratory of Green Pesticide,College of Chemistry,Central China Normal University,Wuhan 430079,China;School of Mechanical and Materials Engineering,Washington State University,WA 99164,USA)

机构地区：[1]华中师范大学化学学院,绿色农药全国重点实验室,智能生物传感技术与健康国际联合研究中心,武汉430079 [2]华盛顿州立大学机械与材料工程学院,美国普尔曼99164

出　　处：《分析化学》2024年第10期1435-1445,共11页Chinese Journal of Analytical Chemistry

基　　金：中央高校基础科研基金项目(No.CCNU22JC006)资助。

摘　　要：水解纳米酶作为一种新型人工酶,具有类水解酶催化活性以及独特的物理化学性质,克服了天然酶在实际应用中的限制,被开发出了新的应用领域。仿生催化的兴起,激发了多种水解纳米酶的设计和开发的研究。然而,由于天然酶催化机制复杂,对其中多种辅因子的精确模拟仍然是一个巨大挑战。本文总结了近年来水解纳米酶的类酶催化及传感应用研究概况,介绍了水解纳米酶仿生设计的最新进展,重点介绍了金属位点和氨基酸微环境两个关键辅因子,概述了水解纳米酶在农药检测、离子检测和生物分子检测等方面的应用,并讨论了水解纳米酶目前面临的机遇和挑战。As a new type of artificial enzyme,hydrolytic nanozymes possess both hydrolase-like catalytic activity and unique physicochemical properties,enabling them to overcome the limitations of natural enzymes in practical applications and foster the development of new application scenarios.The rise of biomimetic catalysis has inspired the design and development of various nanozymes with hydrolase-like activities.However,due to the complexity of the catalytic mechanism of natural enzymes,accurately mimicking multiple cofactors remains a great challenge.This article summarized the latest research progress in nanozymes with hydrolase-like activity for sensing applications.First,inspired by the catalytic mechanism of natural hydrolase,the recent advances in the bionic design of hydrolytic nanozymes were introduced,highlighting two key cofactors:metal sites and amino acid microenvironments.Then,the sensing applications for detection of pesticides,ions,and biomolecules were summarized,and the current opportunities and challenges were described.

关 键 词：纳米酶 仿生催化 类水解酶活性 传感 评述 

分 类 号：TQ426.97[化学工程] TB383.1[一般工业技术—材料科学与工程]