金属元素探针标记策略的研究进展  

Research Progress in Metal Element Probe Tagging Strategies

作　　者：胡悦莉 张程超 李嫣 位艳雪 刘睿[1] 吕弋[1,2] HU Yue-Li;ZHANG Cheng-Chao;LI Yan;WEI Yan-Xue;LIU Rui;LYU Yi(College of Chemistry,Sichuan University,Chengdu 610064,China;Analytical&Testing Centre,Sichuan University,Chengdu 610064,China)

机构地区：[1]四川大学化学学院,成都610064 [2]四川大学分析测试中心,成都610064

出　　处：《分析化学》2024年第10期1457-1464,共8页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(Nos.22074096,22374105,22074098)资助。

摘　　要：金属元素探针标记策略是将金属元素标记在生物分子上并作为信号探针,通过生物识别,联用电感耦合等离子体质谱(ICP-MS)实现对生物分子的定量分析。金属元素标记与ICP-MS技术联用的方法具有灵敏度高、分辨率高和基质效应低等独特优势,拓展了生物分子定量分析的研究思路。金属元素标记策略已被广泛应用于生物分析领域,能够实现灵敏分析和多组分同时检测,并克服了生物分子所处复杂环境产生的干扰,具有良好的应用前景。本文从金属元素探针标记策略出发,系统总结了金属离子标记策略、金属纳米颗粒标记策略、DNA模板化金属纳米颗粒的无标记策略的发展概况,分析了其优缺点,综述了金属元素探针标记策略在生物分子高灵敏度分析和多重检测中的应用研究进展,并展望了其未来的发展趋势。The metal element probe labeling strategy involves marking biomolecules with metal elements as signal-generating units and performing quantitative analysis of biomolecules through inductively coupled plasma mass spectrometry(ICP-MS).The combination of metal element labeling and ICP-MS technology offers significant advantages,including high sensitivity,high resolution,and low matrix effects,thereby expanding the approaches for quantitative analysis of biomolecules.This strategy has been widely applied in bioanalysis for broad purposes including disease diagnosis(or predication)and environmental regulation.Such an approach enables sensitive analysis and simultaneous detection of multiple components while overcoming interference from complex biological environments,presenting substantial application prospects.Over the past two decades,numerous research works have been reported in this area.Based on the abovementioned characteristics of the metal element labeling strategy,in this paper,the development of current labeling strategies based on metal element probe labeling were systematically reviewed,the advantages and disadvantages were discussed,the applications in highsensitivity analysis and multiplex detection of biomolecules were reviewed,and an outlook on future development trends of this technology was provided.

关 键 词：金属元素探针 标记策略 电感耦合等离子体质谱 生物分子 评述 

分 类 号：Q503[生物学—生物化学] O657.63[理学—分析化学]