半导体聚合物和光敏剂掺杂聚苯乙烯微球用于微塑料活体余辉成像示踪  

Semiconducting Polymer and Photosensitizer Co-Doping Polystyrene Microspheres for Afterglow Imaging of Microplastics in Vivo

作　　者：石庭姣 陈丽建 严丽霞 赵旭 严秀平 SHI Ting-Jiao;CHEN Li-Jian;YAN Li-Xia;ZHAO Xu;YAN Xiu-Ping(Institute of Analytical Food Safety,School of Food Science and Technology,Jiangnan University,Wuxi 214122,China)

机构地区：[1]江南大学食品学院,分析食品安全学研究所,无锡214122

出　　处：《分析化学》2024年第10期1517-1527,共11页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(Nos.21934001,22304063);江苏省自然科学基金项目(No.BK20231491)资助。

摘　　要：微塑料对环境和人类健康构成严重威胁,但对于微塑料在生物体内的循环分布及转运过程目前尚未得到充分评估。因此,构建可以成像示踪微塑料的探针,对实现微塑料在机体内循环转运的可视化监测具有重要意义。半导体聚合物不含重金属离子,易生物降解,并且不存在放射性污染,在不持续激发光条件下可获得无组织背景荧光的高信噪比余辉成像,在生物成像领域具有良好的应用前景。本研究以聚苯乙烯微球为微塑料模型、苯乙烯为反应单体、聚乙烯吡咯烷酮为表面活性剂、过硫酸钠为引发剂,在反应过程中将半导体聚合物聚[(9,9-二(2-乙基己基)-9H-氟-烯-2,7-亚乙烯)-共-(1-甲氧基-4-(2-乙基己氧基)-2,5-亚苯基-亚乙烯基)]和光敏剂2,3-萘菁硅二(三己基硅氧烷)进行共同掺杂,合成得到了余辉发光时间长达96 h、组织穿透深度可达到1.8 cm的聚苯乙烯复合微球。研究结果表明,导体聚合物和光敏剂的掺杂对微球的粒径和Zeta电势的影响较小,在小鼠活体内的余辉发光信号可以被持续监测24 h。对解剖后的小鼠器官进行成像发现,灌胃48 h后的小鼠胃部仍可观测到余辉信号。本研究为生物活体内微塑料的成像示踪提供了新方法。Microplastics pose a serious threat to the environment and human health,but the circulating distribution and transport processes of microplastics in organisms have not been fully assessed.Therefore,it is of great significance to construct a probe that can image trace microplastics and visually monitor their circulation and transport in vivo.Afterglow semiconducting polymers are attractive for applications in biological imaging as they do not contain heavy metal ions,are easy to biodegrade and allow high signal-to-noise ratio afterglow imaging without continuous excitation.In this study,with styrene as reaction monomer,polyvinylpyrrolidone as surfactant,and sodium persulfate as initiator,afterglow polystyrene microspheres for in vivo imaging of microplastics were prepared.During the polymerization process,poly[(9,9-di(2-ethylhexyl)-9H-fluo-rene-2,7-vinylene)-co-(1-methoxy-4-(2-ethylhexyloxy)-2,5-phenylenevinylene)](PF-MEHPPV)and photosensitizer silicon 2,3-naphthalocyanine bis(trihexylsilyloxide)(NCBS)were co-doped into polystyrene microspheres.The prepared afterglow polystyrene microspheres exhibited an afterglow duration of 96 h and a tissue penetration depth of 1.8 cm.It was found that co-doping of PF-MEHPPV and NCBS had little effect on the particle size and Zeta potential of the microspheres,and allowed to monitor the afterglow luminescence signal in vivo for 24 h.Imaging of mouse organs after anatomy showed that the afterglow signal was still observed in the stomach of mice 48 h after gavage.This study provided a new method for imaging and tracing microplastics in vivo.

关 键 词：微塑料 聚苯乙烯微球 半导体聚合物 余辉成像 

分 类 号：O657.3[理学—分析化学] X835[理学—化学]