完全性脊髓横断后排尿次数对神经源性膀胱模型大鼠成模率的影响  

Effect of the number of times to urinate on the modeling rate of neurogenic bladder model in rats after complete spinal cord transection

作　　者：梁柔筠 展立芬 曾学究 丁强盛 罗小精 卓越 艾坤[1] 邓石峰 许明[1] 张泓[1] Liang Roujun;Zhan Lifen;Zeng Xuejiu;Ding Qiangsheng;Luo Xiaojing;Zhuo Yue;Ai Kun;Deng Shifeng;Xu Ming;Zhang Hong(College of Acupuncture,Massage and Rehabilitation,Hunan University of Chinese Medicine,Changsha 410208,Hunan)

机构地区：[1]湖南中医药大学针灸推拿与康复学院,湖南省长沙市410208

出　　处：《中国组织工程研究》2025年第18期3840-3847,共8页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金项目(822746668),项目负责人:张泓;国家自然科学基金项目(82205255),项目负责人:许明;湖南省自然科学基金项目(2022JJ40312),项目负责人:许明;湖南中医药大学学科建设揭榜挂帅项目(22JBZ013),项目负责人:张泓;湖南中医药大学研究生创新项目(2023CX13),项目负责人:展立芬~。

摘　　要：背景:脊髓损伤常导致逼尿肌反射亢进型神经源性膀胱,目前对其发病机制和治疗缺乏明确认识,建立稳定可靠的动物模型对揭示疾病的病理机制和探索治疗方法具有重要影响。目的:探究完全性脊髓横断术后辅助排尿次数对神经源性膀胱模型大鼠的影响,以提高神经源性膀胱模型大鼠术后生存率及成模率。方法:从46只雌性SD大鼠中按随机数字表法抽取6只为假手术组,剩余40只大鼠完全性脊髓横断造模后随机分为每日排尿0,1,3,5次组,每组10只。术后19 d内每隔3 d测量残余尿量,术后第19天时观察存活及成模情况,进行尿流动力学检测及离体逼尿肌肌条收缩实验。结果与结论:①存活率及成模率:每日排尿0次组存活率10%,成模率10%;每日排尿1次组存活率20%,成模率10%;每日排尿3,5次组存活率70%,成模率70%;②残余尿量:与假手术组相比,术后第3,6,9,12,15天,每日排尿3,5次组的残余尿量显著增加(P<0.01);术后第18天,每日排尿3,5次组的残余尿量增多(P<0.05);与每日排尿3次组相比,术后第6天,每日排尿5次组的残余尿量减少(P<0.05),术后第3,9,12,15,18天,每日排尿5次组的残余尿量无统计学差异;③尿流动力学:与假手术组相比,每日排尿3,5次组大鼠的漏尿点压差明显减小(P<0.01),膀胱最大容量显著增大(P<0.01);与每日排尿3次组相比,每日排尿5次组的漏尿点压差和膀胱最大容量均无统计学差异;④离体逼尿肌肌条收缩实验:与假手术组相比,每日排尿3,5次组逼尿肌肌条收缩幅度和频率显著减少(P<0.01);与每日排尿3次组相比,每日排尿5次组的逼尿肌肌条收缩幅度和频率均无统计学差异。结果表明:辅助排尿是成功构建逼尿肌反射亢进型神经源性膀胱模型的影响因素之一,每日排尿3次或5次无明显差异,结合实际工作量和成模率,建议每日排尿频率至少3次及以上。BACKGROUND:Spinal cord injury often leads to neurogenic bladder with hyperreflexia of the forced urethral muscle,but there is a lack of clear understanding of its pathogenesis and treatment,and establishing a stable and reliable animal model has an important impact on revealing its pathomechanisms and exploring therapeutic approaches.OBJECTIVE:To investigate the effect of the number of times to urinate on neurogenic model rats after complete spinal cord transection in order to improve the postoperative survival and modeling rate of neurogenic model rats.METHODS:Out of 46 female Sprague-Dawley rats,6 were selected as the sham-operated group using the random number table method,and the remaining 40 rats were randomly divided into 0,1,3,and 5 times daily urination groups after complete spinal cord transection modeling,with 10 rats in each group.The residual urine volume was measured every 3 days within 19 postoperative days,and the survival and modeling were observed on the 19th day after the operation,and urodynamics tests and contraction experiments of isolated forced urethra muscle strips were performed.RESULTS AND CONCLUSION:(1)Survival and modeling rate:there was 10%survival rate and 10%modeling rate in the group of 0 times daily urination;20%survival rate and 10%modeling rate in the group of 1 time daily urination;70%survival rate and 70%modeling rate in the groups of 3 and 5 times daily urination.(2)Residual urine volume:compared with the sham-operated group,the residual urine volume of the groups of 3 and 5 times daily urination was significant increased on postoperative days 3,6,9,12,and 15(P<0.01);the residual urine volume of the groups of 3 and 5 times daily urination was increased on the 18th day after surgery(P<0.05).Compared with the 3 times daily urination group,the residual urine volume was decreased in the 5 times daily urination group on the 6th day after surgery(P<0.05),while there was no significant difference in the residual urine volume between the 3 and 5 times daily urination groups on the 3r

关 键 词：神经源性膀胱 逼尿肌反射亢进型 脊髓损伤 完全性脊髓横断术 排尿次数 手法辅助排尿技术 SD大鼠 工程化动物模型 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学] R587.1