口服卵黄抗体对杂交鳢弹状病毒(HSHRV)感染的免疫保护效果  

Immunoprotective effect of oral yolk antibody against hybrid snakehead rhabdovirus

作　　者：刘田 李硕[2] 汪铭书 赵立宁[2] 黄锦炉 吴艺琳 贾爱卿 LIU Tian;LI Shuo;WANG Mingshu;ZHAO Lining;HUANG Jinlu;WU Yilin;JIA Aiqing(Key Laboratory of Microecological Resources and Utilization in Breeding Industry,Ministry of Agriculture and Rural Affairs,Guangzhou 511400,China;Animal Husbandry and Fisheries Research Center of Guangdong Haid Group Co.,Ltd.,Guangzhou 511400,China;Institute of Preventive Veterinary Medicine,Sichuan Agricultural University,Chengdu 611100,China)

机构地区：[1]农业农村部微生态资源养殖利用企业重点实验室,广东广州511400 [2]广东海大集团股份有限公司畜牧水产研究中心,广东广州511400 [3]四川农业大学预防兽医研究所,四川成都611100

出　　处：《海洋科学》2024年第8期10-16,共7页Marine Sciences

基　　金：2023年乡村振兴战略专项-农业科技发展及资源环境保护管理项目(2023KJ115)。

摘　　要：为了研究抗杂交鳢弹状病毒(hybrid snakehead rhabdovirus,HSHRV)卵黄抗体的制备方法并评价其免疫保护效果,将杂交鳢弹状病毒经白油乳化制备疫苗,免疫蛋鸡收集高免蛋,采用喷雾干燥方法制备成卵黄抗体粉,以细胞中和试验评估卵黄抗体中和病毒的效果。按照每kg鱼投喂卵黄抗体0.25 g、0.40 g、0.60 g的比例制备低、中、高3种添加剂量的饲料,持续投喂杂交鳢10 d,在口服免疫第4天时以104 TCID50/0.1mL的杂交鳢弹状病毒江门I株(HSHRV-JMI)病毒经腹腔注射对杂交鳢进行攻毒,并记录6 d内各组死亡情况。结果显示,四次免疫后卵黄抗体中和效价达到高峰,为1∶46.8,抗体维持时间为60d。高中低剂量组的存活率分别为64.4%、70.6%、78.5%;相对保护率分别为61.1%、67.9%、76.5%,三者均显著高于非特异性卵黄抗体对照组(P<0.001)。表明杂交鳢弹状病毒能有效刺激卵黄抗体产生,高效价卵黄抗体经口服免疫后可以有效抑制杂交鳢弹状病毒的侵染,显著提高杂交鳢存活率和相对保护率。To investigate the preparation method and evaluate the immune protective effect of egg yolk antibodies against hybrid snakehead rhabdovirus(HSHRV),a vaccine with a white oil adjuvant was prepared.Eggs from im-munized laying hens were collected and sprayed to prepare egg yolk antibody powder.The neutralization effect of the egg yolk antibody against the virus was evaluated using a cell neutralization test.Three kinds of immune feed were prepared by adding egg yolk antibody at doses of 0.25,0.4,and 0.6 g per kg of fish.Snakehead hybrids were fed the immune diet for 10 days and challenged with an intraperitoneal injection on the 4th day.Deaths in each group were recorded for 6 days.The results showed that the neutralization titer of the yolk antibody peaked at 1∶46.8 after four immunizations,with an antibody maintenance period of 60 days.The survival rates for the high,middle,and low dose groups were 64.4%,70.6%,and 78.5%,respectively,with relative protection rates of 61.1%,67.9%,and 76.5%,respectively,which were significantly higher than those of the non-specific yolk antibody control group(P<0.001).These results indicate that the neutralization antibody production can be effectively stimulated by HSHRV.Oral immunization with high-titer egg yolk antibodies could effectively inhibit HSHRV infection and sig-nificantly improve the survival and relative protection rates of snakehead hybrids.

关 键 词：杂交鳢弹状病毒(HSHRV) 卵黄抗体 口服免疫 中和效价 免疫保护效果 

分 类 号：S942[农业科学—水产养殖] Q955[农业科学—水产科学] S965[生物学—动物学]