血清HBV RNA分子特征、检测方法及临床应用研究  

Advances in molecular characteristics,detection methods,and clinical applications of serum HBV RNA

作　　者：刘小花[1] 余杨[1] 王桂香[1] 张林艳 陈涛(综述)[1] 黄华翠(审校)[1] LIU Xiaohua;YU Yang;WANG Guixiang;ZHANG Linyan;CHEN Tao;HUANG Huacui(Department of Medical Laboratory,Xindu District People′s Hospital,Chengdu,Sichuan 610500,China)

机构地区：[1]成都市新都区人民医院医学检验科,四川成都610500

出　　处：《国际检验医学杂志》2024年第22期2805-2808,2816,共5页International Journal of Laboratory Medicine

基　　金：成都市医学科研项目(2020201);四川省医学会科研项目(S21094);成都市医学科研项目(2022418)。

摘　　要：肝细胞内共价环状闭合DNA(cccDNA)是乙型肝炎病毒(HBV)的复制中间体,与HBV复制密切相关。同时,它是前基因组RNA(pgRNA)的转录模板,而血清中的HBV RNA多来自未逆转录的pgRNA。近年来许多研究表明HBV RNA在监测疾病进展和预测慢性HBV感染患者的预后等方面有重要作用,是慢性乙型病毒性肝炎的一种潜在的生物标志物。该文从HBV RNA的分子特征、检测方法及临床应用研究进展进行概述。Covalently closed circular DNA(cccDNA)in hepatocytes is the replication intermediate of hepatitis B virus(HBV),which is closely related to HBV replication.Meanwhile,it is the transcriptional template of pregenomic RNA(pgRNA),and most of the HBV RNA in serum is derived from unreversed transcribed pgRNA.In recent years,many studies have demonstrated that HBV RNA has an important role in monitoring disease progression and predicting the prognosis of chronic HBV-infected patients,and is a potential biomarker of chronic viral hepatitis B.This article provides an overview of the molecular characterisation of HBV RNA,detection methods and research progress in clinical applications.

关 键 词：乙型肝炎病毒 乙型肝炎病毒RNA 分子特征 检测方法 临床应用 

分 类 号：R512.62[医药卫生—内科学] R446.6[医药卫生—临床医学]