基于数据挖掘和网络药理学探讨泻心汤类方治疗慢性萎缩性胃炎的用药规律及作用机制  

Exploring the medication rule and action mechanism of the Xiexin decoction in the treatment of chronic atrophic gastritis based on data mining and network pharmacology

作　　者：马力亚 李梅梅 黄玉卓 周会霞 舒劲[2] MA Liya

机构地区：[1]甘肃中医药大学,甘肃兰州730030 [2]甘肃省中医院,甘肃兰州730050

出　　处：《中医临床研究》2024年第28期9-16,共8页Clinical Journal Of Chinese Medicine

基　　金：2023年甘肃中医药大学研究生创新项目。

摘　　要：目的:探究泻心汤类方治疗慢性萎缩性胃炎(Chronic Atrophic Gastritis,CAG)的用药规律及作用机制。方法:检索中国知网、万方数据等平台相关文献,建立中药处方数据库,利用中医传承辅助平台总结用药规律,得出核心角药。利用中药系统药理学数据库与分析平台(TCMSP)检索药物主要有效成分及靶点,使用GeneCards数据库和OMIM数据库搜索CAG疾病靶点,取有效成分与疾病靶点的交集,绘制韦恩图。利用Cytoscape将药物-活性成分-交集靶点调控网络可视化。基于R语言进行基因本体论(GO)功能和京都基因与基因组百科全书(KEGG)通路富集分析。结果:共纳入195篇文献,涉及199首方剂,其中使用频次大于20次的高频药物有19味,加减药物以理气药、活血化瘀药为主。核心角药为“半夏-黄芩-黄连”,其有效成分总靶点203个、疾病靶点1059个,二者交集靶点83个。有效成分以黄芩素、槲皮素、刺槐素为主。经蛋白质-蛋白质相互作用网络分析,关键靶点有肿瘤蛋白p53(Tumor Protein p53,TP53)、丝氨酸/苏氨酸蛋白激酶1(Akt Serine/Threonine Kinase 1,AKT1)、肿瘤坏死因子(Tumor Necrosis Factor,TNF)、白细胞介素(Interleukin,IL)-6、B细胞淋巴瘤-2蛋白(B-cell lymphoma-2,BCL2)等,通过GO功能富集分析得到2160个条目,涵盖生物过程、细胞组成及分子功能,通过KEGG通路富集分析得到159条信号通路。结论:在核心角药“半夏-黄芩-黄连”治疗CAG中发挥关键作用的活性成分为黄芩素、槲皮素、刺槐素等,关键靶点为TP53、AKT1、TNF、IL-6、BCL2等,其作用主要与磷脂酰肌醇3激酶(Phosphatidylinositol 3-kinase,PI3K)-蛋白激酶B(Akt)信号通路、IL-17信号通路、TNF信号通路、细胞凋亡通路及癌症相关通路等有关,可见泻心汤类方治疗CAG具有多成分、多靶点、多通路的特点,为泻心汤类方治疗CAG提供了理论基础。Objective:To explore the medication rule and action mechanism of the Xiexin decoction(泻心汤)in the treatment of chronic atrophic gastritis(CAG).Methods:Relevant literature was retrieved from CNKI and Wanfang databases to establish a database.Traditional Chinese medicine inheritance support system was used to analyze and summarize medication rules,and obtain core tripartite medicines.Traditional Chinese medicine systems pharmacology database and analysis platform was used to retrieve the main active ingredients and targets,and GeneCards database and OMIM database were used to search for CAG disease targets.The intersection of the active components and the disease targets was taken to draw the Venn diagram.Visualization of medicine-active ingredient-intersection target regulatory network was carried out in Cytoscape.GO function and KEGG signal pathway enrichment analyses were performed by R language software.Results:A total of 195 articles were included,involving 199 prescriptions,among which 19 high-frequency medicines were used more than 20 times.The modified drugs were mainly Qi(气)-regulating drugs,blood-activating and stasis-removing drugs,etc..The core tripartite medicines were Banxia(Rhizoma Pinelliae)-Huangqin(Radix Scutellariae)-Huanglian(Rhizoma Coptidis),with 203 active ingredient targets,1059 disease targets,and 83 intersecting targets.The main active ingredients were baicalin,quercetin,and locutin.According to protein-protein interaction network analysis,key targets included TP53,AKT1,TNF,IL-6,BCL2,etc..Through GO function enrichment analysis,2160 entries were obtained,including the biological processes,cellular components,and molecular functions.KEGG pathway enrichment analysis revealed 159 signaling pathways.Conclusions:It was concluded that the key active ingredients of the core tripartite medicines Banxia-Huangqin-Huanglian in the treatment of CAG were baicalin,quercetin,locutin,etc.,that the key targets were TP53,AKT1,TNF,IL-6,BCL2,etc.,and that its role is mainly related to PI3K-Akt signalin

关 键 词：慢性萎缩性胃炎 泻心汤类方 数据挖掘 用药规律 机制研究 

分 类 号：R256.3[医药卫生—中医内科学]