直接回流法提取头发中辅酶Q_(10)的工艺研究  

Study on Extraction of Coenzyme Q_(10) from Hair by Direct Reflux Method

作　　者：吕思敏 丁鸿燕 李明贵 吴铁 LYU Simin;DING Hongyan;LI Minggui;WU Tie(Guangdong Medical University·Guangdong Runhe Biological Technology Co.,Ltd.,Coenzyme Q_(10)Research Institute,Dongguan 523808,China;School of Pharmacy,Guangdong Medical University,Dongguan 523808,China)

机构地区：[1]广东医科大学广东润和生物科技有限公司辅酶Q_(10)联合研究中心,广东东莞523808 [2]广东医科大学药学院,广东东莞523808

出　　处：《山东化工》2024年第20期60-63,共4页Shandong Chemical Industry

基　　金：广东医科大学·广东润和生物科技有限公司共建辅酶Q_(10)联合研究中心项目(2XX14002)。

摘　　要：采用直接回流的工艺提取头发中的辅酶Q_(10),研究不同的提取条件对辅酶Q_(10)提取量的影响,并采用正交试验对提取液的料液比,温度,时间进行优化,确定头发中辅酶Q_(10)的最佳提取条件。结果表明,以无水乙醇为提取试剂,其直接回流的最佳提取条件为料液比1∶10(g∶mL),温度80℃,时间为120 min。在此条件下,头发中提取的辅酶Q_(10)最大量为0.726 3μg/g,RSD为1.86%;辅酶Q_(10)的平均回收率为94.21%,RSD为4.42%。Direct reflux process was used to extract coenzyme Q_(10)from hair,and the influence of different extraction conditions on the extraction amount of Coenzyme Q_(10) was studied.Orthogonal test was used to optimize the solid-liquid ratio,temperature and time of the extraction solution,so as to determine the best extraction conditions of coenzyme Q_(10) from hair.The results showed that the optimal extraction conditions were solid-liquid ratio 1∶10(g∶mL),temperature 80℃ and time 120 min with anhydrous ethanol as extraction reagent.Under these conditions,the maximum amount of coenzyme Q_(10) extracted from hair was 0.7263μg/g,and the RSD was 1.86%.The average recovery of Coenzyme Q_(10) was 94.21% and RSD was 4.42%.

关 键 词：直接回流 头发 辅酶Q_(10) 高效液相色谱法 

分 类 号：TQ460[化学工程—制药化工] R284.1[医药卫生—中药学]