硒化当归多糖对巨噬细胞功能的影响及机制  

Effects and mechanisms of macrophage function by selenizing Chinese angelica polysaccharide

作　　者：乔杰 海尼斯格 高瑞峰 杨英[1] 高珍珍 QIAO Jie;HAINI Sige;GAO Ruifeng;YANG Ying;GAO Zhenzhen(College of Veterinary Medicine,Inner Mongolia Agricultural University,Hohhot 010018,China;College of Animal Husbandry and Veterinary Medicine,Jiangsu Vocational College of Agriculture and Forestry,Jurong,Jiangsu 212499,China)

机构地区：[1]内蒙古农业大学兽医学院,内蒙古呼和浩特010018 [2]江苏农林职业技术学院畜牧兽医学院,江苏句容212499

出　　处：《中国兽医学报》2024年第9期1999-2009,共11页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(31960714)。

摘　　要：旨在探讨当归多糖(Chinese angelica polysaccharide,CAP)硒化前后对腹腔巨噬细胞功能的影响。试验将当归多糖和硒化当归多糖分别作用于小鼠腹腔巨噬细胞,用流式细胞术检测巨噬细胞的吞噬活性以及细胞表型F4/80、MHC-Ⅱ、CD80和CD86的表达量,并用ELISA检测巨噬细胞上清液中IL-6、IL-10、NO、MIP-1α和TNF-α含量变化。试验显示:sCAP质量浓度在1.96 mg/L时,腹腔巨噬细胞的吞噬活性显著高于其他各组(P<0.05);F4/80、MHC-Ⅱ、CD80和CD86的表达量显著高于CAP组和细胞对照组(P<0.05);质量浓度为7.81~1.96 mg/L时,巨噬细胞上清液中IL-6、IL-10、NO、MIP-1α的含量显著高于其他各组(P<0.05)。结果表明,sCAP对腹腔巨噬细胞免疫功能有增强作用,且以质量浓度1.96 mg/L最佳。The aim of this experiment was to investigate the effects of Chinese angelica polysaccharide(CAP)on peritoneal macrophage function before and after selenization.In the experiment,CAP and selenized Chinese angelica polysaccharide(sCAP)were applied to mouse peritoneal macrophages,and the phagocytic activity of macrophages as well as the expression of the cellular phenotypes F4/80,MHC-Ⅱ,CD80 and CD86 were detected by flow cytometry,and the changes of IL-6,IL-10,NO,MIP-1α,and TNF-αcontent in the supernatant of macrophage were detected by ELISA.The results showed that the phagocytic activity of peritoneal macrophages was significantly higher in the sCAP mass concentration of 1.96 mg/L than in the other groups(P<0.05);and the expression of F4/80,MHC-Ⅱ,CD80 and CD86 were significantly higher in the sCAP mass concentration of 1.96 mg/L than in the CAP group and cell control group(P<0.05);the contents of IL-6,IL-10,NO,and MIP-1αin the supernatant of macrophages were significantly higher than those in other groups when the mass concentration of sCAP ranged from 7.81 to 1.96 mg/L(P<0.05).The results showed that sCAP had an enhancing effect on the immunomodulatory function of abdominal macrophages and was optimal at a mass concentration of 1.96 mg/L.

关 键 词：硒化当归多糖 巨噬细胞 吞噬活性 细胞因子 

分 类 号：S853.75[农业科学—临床兽医学]