邻菲罗啉对大肠杆菌生物被膜的抑制作用  

Effect of 1,10-phenanthrone monohydrate on biofilm formation in Escherichia coli

作　　者：王瑜 武周慧 杜衡 肖爽 鲁琳[1] 王真[1] WANG Yu;WU Zhouhui;DU Heng;XIAO Shuang;LU Lin;WANG Zhen(Beijing Key Laboratory of Traditional Chinese Veterinary Medicine,Animal Science and Technology College,Beijing University of Agriculture,Beijing 102206,China)

机构地区：[1]北京农学院动物科学技术学院兽医学(中兽医)北京市重点实验室,北京102206

出　　处：《中国兽医学报》2024年第9期2017-2024,2071,共9页Chinese Journal of Veterinary Science

基　　金：2023年北京农学院学位与研究生教育改革与发展基金资助项目(5056516030/003);北京市优秀人才培养基金资助项目(2017000026833ZK18);现代农业产业技术体系北京市创新团队基金资助项目(BAIC08-2023);北京农学院青年科学基金资助项目(QNKJ202107)。

摘　　要：探究邻菲罗啉对大肠杆菌抗生物被膜活性,初步分析其对大肠杆菌的抗膜机制。通过测定时间杀菌曲线评价邻菲罗啉对大肠杆菌的抑菌效果,测定邻菲罗啉对大肠杆菌最小生物被膜抑制浓度(minimal biofilm inhibitory concentration,MBIC);通过荧光显微镜观察邻菲罗啉对大肠杆菌生物被膜的抑制作用;检测邻菲罗啉对大肠杆菌生物被膜发育阶段及EPS的影响。结果显示,邻菲罗啉对大肠杆菌的抑制作用呈浓度依赖性,对大肠杆菌E-10的MBIC值为12.5μmol/L,50.0μmol/L即可显著破坏大肠杆菌成熟生物被膜,邻菲罗啉在生物被膜形成初期通过抑制大肠杆菌的黏附能力抑制生物被膜的形成。邻菲罗啉可抑制大肠杆菌生物被膜EPS的合成与分泌,1/4最低抑菌浓度(minimum inhibitory concentration,MIC)浓度对胞外多糖的抑制率分别为32%和78%,1/4 MIC浓度下邻菲罗啉对ATCC 29522和E-10的eDNA的抑制率分别为72%和61%,MIC浓度下邻菲罗啉对胞外蛋白的抑制率分别为69%和56%。邻菲罗啉对大肠杆菌的具有抗生物被膜活性,为寻找抗生素替代药物提供了新思路。The aim of this study is to investigate the anti-biofilm activity of 1,10-PHEN against E.coli and to analyze its anti-membrane mechanism.The bacteriostatic effect of 1,10-PHEN on E.coli was evaluated by measuring the time bactericidal curve,and the minimum biofilm inhibitory concentration(MBIC)of 1,10-PHEN on E.coli was measured.The inhibitory effect of 1,10-PHEN on E.coli biofilm under fluorescence microscope was observed.The effects of 1,10-PHEN on the development stage and EPS of E.coli biofilm were detected.The inhibitory effect of 1,10-PHEN on E.coli was concentration-dependent.The MBIC values of E.coli E-10 were 12.5μmol/L,and 50.0μmol/L could significantly destroy the mature biofilm of E.coli.1,10-PHEN could inhibit the formation of biofilm by inhibiting the adhesion ability of E.coli at the early stage of biofilm formation.1,10-PHEN could inhibit the synthesis and secretion of EPS from E.coli biofilm.1,10-PHEN could inhibit the synthesis and secretion of EPS from E.coli biofilm,and the inhibition rates of EPS at 1/4 MIC were 32%and 78%,respectively.The inhibitory rates of 1,10-PHEN on eDNA of ATCC 29522 and E-10 at 1/4 MIC concentration were 72%and 61%,respectively,and the inhibitory rates of MIC concentration on extracellular protein were 69%and 56%.In conclusion,1,10-PHEN has anti-biofilm activity against E.coli,which provides a new idea for finding alternative antibiotics.

关 键 词：邻菲罗啉 大肠杆菌 生物被膜 抗菌活性 作用机制 

分 类 号：S852.61[农业科学—基础兽医学]