慢性冷暴露对小鼠肾脏的影响  

Effects of chroniccold exposure on mice kidney

作　　者：张嘉俊 计红[1] 徐彬 ZHANG Jiajun;JI Hong;XU Bin(College of Animal Science and Veterinary Medicine,Heilongjiang Bayi Agricultural University,Daqing,Heilongjiang 163319,China)

机构地区：[1]黑龙江八一农垦大学动物科技学院,黑龙江大庆163319

出　　处：《中国兽医学报》2024年第9期2050-2054,共5页Chinese Journal of Veterinary Science

基　　金：黑龙江省自然科学基金资助项目(YQ2021C027);“三纵”青年创新人才计划基金资助项目(ZRCQC202003)。

摘　　要：为探究慢性冷暴露下对小鼠肾脏造成的影响。将5周龄C57BL/6小鼠随机分为常温对照组和冷暴露组,常温对照组于(24±2)℃的室温环境饲养,冷暴露组小鼠置于4℃人工智能气候室每日08:00—20:00随机选择3 h刺激,连续刺激3周,本研究应用蛋白免疫印迹检测了小鼠肾脏中氧化应激及细胞凋亡相关蛋白的表达。结果显示,冷暴露后小鼠肾脏Nrf-2、HO-1、Cyt-c、BAX/Bcl-2及活化的Caspase-3蛋白表达明显升高(P<0.001);而Keap-1蛋白表达显著降低(P<0.001)。结果表明,冷暴露可加剧小鼠肾脏的氧化应激,并诱导细胞凋亡的发生,对肾脏造成一定的损伤。本研究为深入探究慢性冷暴露造成的肾脏损伤机制提供了参考,为畜牧业养殖过程中因冷暴露导致的疾病提供了一定的理论基础。This experiment is to explore the effect of chronic cold exposure on the mouse kidney.C57BL/6 mice aged 5 weeks were randomly divided into a normal temperature control group and a cold exposure group.The normal temperature control group was fed at room temperature of(24±2)℃,and the cold exposure group was placed in a 4℃artificial intelligence climate room.The mice were randomly stimulated for 3 hours from 8 a.m.to 8 p.m.every day for three weeks.In this study,Western blot was used to detect the expression of oxidative stress and apoptosis related proteins in mouse kidneys.The results showed that after cold exposure,the expressions of Nrf-2,HO-1,Cyt-C,BAX/Bcl-2 and activated Caspase-3 protein in mouse kidney increased significantly(P<0.001).The expression of Keap-1 protein decreased significantly(P<0.001).The results showed that cold exposure could aggravate the oxidative stress of the mouse kidney,induce apoptosis,and cause some damage to the kidney.This result provides a reference for further exploring the mechanism of kidney damage caused by chronic cold storms,and provides a theoretical basis for diseases caused by cold exposure in the process of animal husbandry.

关 键 词：冷暴露 肾脏 氧化应激 细胞凋亡 

分 类 号：S852.2[农业科学—基础兽医学]