B族链球菌耐药性、耐药基因及MLST分型分析  

Analysis of antibiotic resistance,antibiotic resistance genes and MLST typing of group B streptococcus

作　　者：杨琰琰 王晓伟 王泽华 杜慧贞 YANG Yanyan;WANG Xiaowei;WANG Zehua;DU Huizhen(Department of Clinical Laboratory,Qilu Hospital of Shandong University(Qingdao),Qingdao,Shandong,China,266035;Qingdao Norson Biotech Co.,Ltd,Qingdao,Shandong,China,266736)

机构地区：[1]山东大学齐鲁医院(青岛)检验科,山东青岛266035 [2]青岛诺森生物技术有限责任公司,山东青岛266736

出　　处：《分子诊断与治疗杂志》2024年第11期2077-2081,共5页Journal of Molecular Diagnostics and Therapy

基　　金：山东省自然科学基金青年项目(ZR2021QH322)。

摘　　要：目的调查青岛地区孕晚期妇女B族链球菌(GBS)耐药性、耐药基因及MLST分型特点,为临床提供参考。方法收集2021年1月至12月山东大学齐鲁医院(青岛)的孕晚期妇女阴道⁃直肠拭子1573份,分离培养后进行菌株鉴定和药敏试验,然后对其耐药基因和管家基因进行扩增、测序,与MLST官网数据库进行比对获得ST分型。结果分离鉴定出GBS菌株63株,定植率为4.01%,对青霉素、氨苄西林、利奈唑胺、万古霉素均为敏感;红霉素耐药菌株中ermB检出率最高,为80.00%,克林霉素耐药菌株中linB被检出,为6.38%;MLST分型鉴定出16种ST型,其中ST10占比最高,为33.33%,且不同MLST分型菌株对四环素、左氧氟沙星、莫西沙星的耐药率比较差异有统计学意义(χ^(2)=33.221、49.137、49.137,P<0.05),不同MLST分型菌株对耐药基因ermB、ermC的携带率比较差异有统计学意义(χ^(2)=16.504、25.705,P<0.05),不同MLST分型菌株之间的多重耐药率比较差异具有统计学意义(χ^(2)=18.858、31.819、26.706,P<0.05)。结论青岛地区孕晚期妇女GBS的定植率在全国处于偏低水平,但GBS菌株多重耐药比例较高。青霉素、氨苄西林仍然可作为防治孕晚期妇女GBS感染的一线药物,对青岛地区临床GBS合理用药具有重要的借鉴意义。Objective To investigate characteristics of antibiotic resistance,antibiotic resistance genes and MLST typing of group B streptococcus(GBS)in late pregnant women in Qingdao as a source of clinical reference.Methods 1,573 vaginal⁃rectal swabs were collected from late pregnant women at Qilu Hospital of Shandong University(Qingdao)from January to December 2021.After isolation and culture,the strains were identified and underwent antimicrobial susceptibility testing.The antibiotic resistance genes and housekeeping genes of GBS strains were amplified and sequenced,then compared with the MLST database to obtain ST typing.Results A total of 63 strains of GBS were isolated and identified,resulting in a coloniza⁃tion rate of 4.01%.All screened strains were found to be sensitive to penicillin,ampicillin,linezolid,and vancomycin.The detection rate of ermB in erythromycin⁃resistant strains was 80.00%,the highest among all detection rates.LinB was detected in clindamycin⁃resistant strains at a rate of 6.38%.Through MLST typing,a total of 16 ST types were identified.the most common ST typing was ST10,accounting for 33.33%of the total.There were statistically significant differences in resistance rates to tetracycline,levofloxacin,and moxifloxacin among different MLST types(χ^(2)=33.221,49.137,49.137,P<0.05).The carrier rates of the antibiotic re⁃sistance genes ermB and ermC also showed statistically significant differences among different MLST typings(χ^(2)=16.504,25.705,P<0.05).Additionally,there were statistically significant differences in multidrug resistance rates among different MLST typings(χ^(2)=18.858,31.819,26.706,P<0.05).Conclusion The colonization rate of GBS in late pregnant women in Qingdao was low compared to other regions in the country.However,the proportion of multidrug⁃resistant GBS strains in this area was high.Despite this,penicillin and ampicillin could still be used as first⁃line drugs for preventing and treating GBS infection in late pregnant women.This information has significant impli

关 键 词：B族链球菌 耐药性 耐药基因 MLST分型 

分 类 号：R446.5[医药卫生—诊断学]