不同超高效液相系统对色谱柱柱效能测定结果的影响  

The influence of different ultra⁃high performance liquid chromatography systems on the determination results of chromatographic column efficiency

作　　者：徐苏华[1] 邱雪梅 卢翰生 林山云 陈华燕 何嘉丽 XU Suhua;QIU Xuemei;LU Hansheng;LIN Shanyun;CHEN Huayan;HE Jiali(Guangdong Medical Devices Quality Surveillance and Test Institute,Guangzhou,Guangdong,China,51066;Shenzhen BGI Biomedical Engineering Co.,Ltd.,Shenzhen,Guangdong,China,518083)

机构地区：[1]广东省医疗器械质量监督检验所,广东广州510663 [2]深圳华大基因生物医学工程有限公司,广东深圳518083

出　　处：《分子诊断与治疗杂志》2024年第11期2082-2085,共4页Journal of Molecular Diagnostics and Therapy

基　　金：广东省药品监督管理局科技创新项目(2023YDZ08)。

摘　　要：目的探索GB/T 30433⁃2021中柱效能的测试参数,是否适用于高效液相色谱柱。方法比较同一超高效液相系统下,不同甲醇/水溶液流动相比例(40∶60,50∶50,55∶45),不同流量(0.20 mL/min,0.22 mL/min,0.25 mL/min,0.28 mL/min,0.30 mL/min),不同进样量(0.1μL,0.2μL)对高效液相色谱柱柱效能的影响;比较两种超高效液相系统(1290 Infinity II和APUS Plus),在两种不同色谱参数(色谱参数一∶乙腈/水溶液=40∶60,流量=0.5 mL/min,进样量=1.0μL;色谱参数二∶甲醇/水溶液=50∶50,流量=0.20 mL/min,进样量=0.2μL)下,对高效液相色谱柱柱效能每米理论板数的影响。结果同一超高效液相系统,在甲醇/水溶液比例为50∶50,流量为0.20 mL/min,进样量为0.2μL的色谱参数下,高效液相色谱柱柱效能每米理论板数的结果较优;不同超高效液相色谱系统下每米理论板数测试结果差异较大。结论不同超高效液相系统下,柱效能每米理论板数的测定结果除受色谱柱自身性能和色谱参数的影响,还与系统及检测器等柱外硬件相关。Objective To explore whether the test parameters for column efficiency in GB/T 30433⁃2021 are applicable to high⁃performance liquid chromatography columns.Methods The effects of different methanol/water solution mobile phase ratios(40∶60,50∶50,55∶45),flow rates(0.20 mL/min,0.22 mL/min,0.25 mL/min,0.28 mL/min,0.30 mL/min),and injection volumes(0.1μL,0.2μL)on the column efficiency of high⁃performance liquid chromatography under the same ultra⁃high performance liquid chromatography system were compared.The effects of two ultra⁃high performance liquid chromatography systems(1290 InfinityⅡand APUS Plus)on the theoretical number of plates per meter in high⁃performance liquid chromatography under two different chromatographic parameters(Chromatographic parameter 1:acetonitrile/aqueous solution=40∶60,flow rate=0.5 mL/min,injection rate=1.0μL;Chromatographic parameter 2:ethanol/aqueous solution=50∶50,flow rate=0.20 mL/min,injection rate=0.2μL)were evaluated.Results Under the same ultra⁃high performance liquid chromatography system,with a methanol/water solution ratio of 50:50,a flow rate of 0.20 mL/min,and an injection volume of 0.2μL,the column efficiency per meter of theoretical plates is improved.The test results show that the theoretical plates per meter vary greatly under different ultra⁃high performance liquid chromatography systems.Conclusion The determination of theoretical plate number per meter of column efficiency in different ultra⁃high performance liquid chromatography systems is influenced not only by the performance of the chromatographic column itself and chromatographic parameter conditions,but also by the system and detector hardware outside the column.

关 键 词：色谱柱 超高效液相色谱 柱效能 理论板数 每米理论板数 

分 类 号：O657.72[理学—分析化学]