子宫内膜异位症患者外周血中miR⁃214和TGF⁃β水平变化及临床意义  

Changes of miR⁃214 and TGF⁃βlevels in peripheral blood of patients with endometriosis and their clinical significance

作　　者：布海切木·卡德尔[1] 陈立乔 陈志芳[1] BUHAIQIEMU Kadeer;CHEN Liqiao;CHEN Zhifang(Department of Gynecology,the First Affiliated Hospital of Xinjiang Medical University,Urumqi,Xinjiang,China,830054)

机构地区：[1]新疆医科大学第一附属医院妇科,新疆乌鲁木齐830054

出　　处：《分子诊断与治疗杂志》2024年第11期2139-2143,共5页Journal of Molecular Diagnostics and Therapy

基　　金：新疆维吾尔自治区自然科学基金项目(2018D01C185)。

摘　　要：目的探究子宫内膜异位症(EMS)患者外周血中微小核糖核酸(miR)⁃214和转化生长因子⁃β(TGF⁃β)水平变化及临床意义。方法选取2022年1月到2023年6月期间中国新疆医科大学第一附属医院收治的116例有生育需求的EMS患者,设为EMS组,根据美国生殖医学会分期标准(r⁃AFS)分为轻度组(n=42)、中度组(n=44)和重度组(n=30),另选取同期于本院体检的健康女性为健康组(n=40),比较4组外周血miR⁃214和TGF⁃β水平;采用Spearman法分析外周血miR⁃214、TGF⁃β水平与EMS严重程度的相关性。另根据EMS患者治疗出院后自然妊娠结局随访分为自然妊娠成功组(n=76)和自然妊娠失败组(n=40),采用多因素Logistic分析外周血miR⁃214和TGF⁃β水平与EMS患者自然妊娠失败的关系,并以受试者工作特性曲线(ROC)分析外周血miR⁃214和TGF⁃β水平对EMS患者自然妊娠失败的预测效能。结果miR⁃214表达比较,健康组>轻度组>中度组>重度组(F=14.894,均P<0.001);TGF⁃β表达情比较,健康组<轻度组<中度组<重度组(F=11.863,均P<0.001);Spearman相关分析显示,EMS严重程度与外周血miR⁃214水平呈负相关(r=-0.481,P<0.001),与TGF⁃β水平呈正相关(r=0.532,P<0.001);多因素回归分析显示,r⁃AFS为Ⅲ~Ⅳ期,外周血miR⁃214水平降低、TGF⁃β水平升高均为EMS患者自然妊娠失败的独立危险因素(P<0.05);ROC曲线分析显示,miR⁃214、TGF⁃β以及二者联合检测的曲线下面积(AUC)分别为0.839、0.790以及0.900,且联合检测预测价值最佳。结论EMS患者外周血中miR⁃214和TGF⁃β水平均出现明显的表达紊乱,并均与EMS患者病情进展密切关联,且二者联合检测EMS患者自然妊娠失败的预测效能较高。Objective To investigate the changes of micrornas(Mir⁃214)and transforming growth factor⁃β(TGF⁃β)levels in peripheral blood of patients with endometriosis(EMS)and their clinical significance.Methods A total of 116 EMS patients with fertility needs admitted to the First Affiliated Hospital of Xinjiang Medical University in China from January 2022 to June 2023 were selected for the EMS group.They were divided into three groups based on severity:mild(n=42),moderate(n=44)and severe(n=30)according to the staging criteria(r⁃AFS)set by the American Society for Reproductive Medicine.Healthy women who underwent physical examinations at our hospital during the same period were chosen as the healthy group(n=40).The levels of miR⁃214 and TGF⁃βin peripheral blood were compared among the 4 groups.The Spearman method was used to analyze the correlation between miR⁃214 and TGF⁃βlevels in peripheral blood and the severity of EMS.In addition,EMS patients were categorized into two groups based on natural pregnancy outcomes post⁃discharge:the natural pregnancy success group(n=76)and the natural pregnancy failure group(n=40).Multivariate logistic analysis was conducted to explore the relationship between miR⁃214 and TGF⁃βlevels in peripheral blood and natural pregnancy failure in EMS patients.The predictive efficacy of peripheral blood miR⁃214 and TGF⁃βlevels on spontaneous pregnancy failure in EMS patients was assessed using a receiver operating characteristic curve(ROC).Results The expression of miR⁃214 was compared among the healthy group,mild group moderate group,and severe group(F=14.894,P<0.001).TGF⁃βexpression was also compared among these groups,showing a pattern of healthy group<mild group<moderate group<severe group(F=11.863,P<0.001).Spearman correlation analysis revealed a negative correlation between EMA severity and miR⁃214 levels in peripheral blood(r=-0.481,P<0.001),and a positive correlation with TGF⁃βlevels(r=0.532,P<0.001).Multifactor regression analysis showed that r⁃AFS

关 键 词：子宫内膜异位症 临床意义 转化生长因子⁃β 自然妊娠 微小核糖核酸⁃214 

分 类 号：R711.71[医药卫生—妇产科学]