不同产地伸筋草的质量评价  

Quality evaluation of Lycopodium japonicum from different producing areas

作　　者：张蕊 冯晓川 徐延昭[4] 许保海 ZHANG Rui;FENG Xiaochuan;XU Yanzhao;XU Baohai(Dept.of Chinese Pharmacy,Beijing Jishuitan Hospital,Capital Medical University,Beijing 100035,China;The Fourth Clinical Medical College of Peking University,Beijing 100035,China;National Center for Orthopedics,Beijing 100035,China;National Institutes for Food and Drug Control,Beijing 100050,China)

机构地区：[1]首都医科大学附属北京积水潭医院中药房,北京100035 [2]北京大学第四临床医学院,北京100035 [3]国家骨科医学中心,北京100035 [4]中国食品药品检定研究院,北京100050

出　　处：《中国药房》2024年第22期2732-2738,共7页China Pharmacy

基　　金：国家中医药管理局2023年全国中药特色技术传承人才培训项目(No.国中医药人教函〔2023〕96号);北京市医院管理中心“青苗”计划(No.QML20230407);北京市属医院科研培育计划(No.PZ2021005);北京积水潭医院高层次人才“学科骨干”计划专项(No.XKGG202214)。

摘　　要：目的评价不同产地伸筋草的质量。方法以6省产的16批伸筋草为样品,采用一测多评(QAMS)法检测伸筋草中α-玉柏碱、β-玉柏碱、石松碱、石松灵碱、伸筋草萜宁醇、21-表千层塔烯二醇、豆甾醇和β-谷甾醇含量,并与外标法结果进行比较;根据《中国药典》附录方法检测浸出物和总灰分。将上述指标结合主成分分析、正交偏最小二乘法-判别分析、加权逼近理想排序(TOPSIS)法评价其质量。结果16批样品中伸筋草萜宁醇含量为0.183~0.446 mg/g;以伸筋草萜宁醇为内参物,按QAMS法测得α-玉柏碱、β-玉柏碱、石松碱、石松灵碱、21-表千层塔烯二醇、豆甾醇和β-谷甾醇的含量分别为1.250~2.097、0.690~1.184、0.035~0.102、0.076~0.150、0.356~0.570、0.085~0.229、0.238~0.855 mg/g,与外标法结果无显著差异;浸出物为12.18%~22.78%,总灰分为3.16%~6.11%。主成分分析显示S1~S6、S7~S11、S12~S16分别聚为3类。α-玉柏碱、β-谷甾醇、伸筋草萜宁醇、β-玉柏碱和21-表千层塔烯二醇的变量重要性投影值均大于1。加权TOPSIS法评价结果显示,S14样品的质量最优(相对贴近度为0.7092)。结论云南绥江县伸筋草质量最优。本研究建立的QAMS法结合化学计量学和加权TOPSIS分析可用于不同产地伸筋草的质量评价。OBJECTIVE To evaluate the quality of Lycopodium japonicum from different producing areas.METHODS Sixteen batches of L.japonicum from six provinces were used as samples.The contents ofα-obscurine,β-obscurine,lycopodine,lycodoline,lyclaninol,21-episerratenediol,stigmasterol andβ-sitosterol in L.japonicum were detected by quantitative analysis of multi-components by single-marker(QAMS).The results were compared with the results of external standard method.The extract and total ash were measured according to the appendix of Chinese Pharmacopoeia.Principal component analysis,orthogonal partial least squares-discriminant analysis and weighted technique for order preference by similarity to ideal solution(TOPSIS)methods were combined to evaluate its quality.RESULTS The contents of lyclaninol in the 16 batches of L.japonicum were 0.183-0.446 mg/g.Using lyclaninol as internal reference material,the contents ofα-obscurine,β-obscurine,lycopodine,lycodoline,21-episerratenediol,stigmasterol andβ-sitosterol were 1.250-2.097,0.690-1.184,0.035-0.102,0.076-0.150,0.356-0.570,0.085-0.229 and 0.238-0.855 mg/g,respectively,with no significant difference from the results of external standard method.The extract was 12.18%-22.78%,and the total ash was 3.16%-6.11%.Principal component analysis showed that samples S1-S6,S7-S11,S12-S16 could be clustered into 3 categories.The variable importance in projection values forα-obscurine,β-sitosterol,lyclaninol,β-obscurine and 21-episerratenediol were all greater than 1.The weighted TOPSIS evaluation results showed that sample S14 had the best quality(Jb=0.7092).CONCLUSIONS L.japonicum from Suijiang county of Yunnan province had the best quality.The established QAMS combined with chemometric and weighted TOPSIS methods can be used for quality evaluation of L.japonicum from different producing areas.

关 键 词：伸筋草 高效液相色谱法 化学计量学方法 正交偏最小二乘判别分析法 加权TOPSIS法 质量评价 一测多评法 

分 类 号：R917[医药卫生—药物分析学] R284[医药卫生—药学]