Thalamic Nucleus Reuniens Glutamatergic Neurons Mediate Colorectal Visceral Pain in Mice via 5-HT2B Receptors  

作　　者：Di Li Han Du Shu-Ting Qu Jing-Lai Wu Yong-Chang Li Qi-Ya Xu Xia Chen Xiao-Xuan Dai Ji-Tian Xu Qian Wang Guang-Yin Xu 

机构地区：[1]Jiangsu Key Laboratory of Neuropsychiatric Diseases and Institute of Neuroscience,Soochow University,Suzhou,215123,China [2]Department of Gastroenterology,The First Affiliated Hospital of Soochow University,Suzhou,215123,China [3]Department of Anesthesiology,Children’s Hospital of Soochow University,Suzhou,215123,China [4]Department of Physiology and Neurobiology,College of Basic Medical Sciences,Zhengzhou University,Zhengzhou,450001,China

出　　处：《Neuroscience Bulletin》2024年第10期1421-1433,共13页神经科学通报（英文版）

基　　金：supported by grants from the National Natural Science Foundation of China (81920108016 and 32230041);the Priority Academic Program Development of Jiangsu Higher Education Institutions of China;the Chinese Red Cross Foundation National Brain Nutrition Research Fund.

摘　　要：Irritable bowel syndrome(IBS)is a common functional bowel disorder characterized by abdominal pain and visceral hypersensitivity.Reducing visceral hypersensitivity is the key to effectively relieving abdominal pain in IBS.Increasing evidence has confirmed that the thalamic nucleus reuniens(Re)and 5-hydroxytryptamine(5-HT)neurotransmitter system play an important role in the development of colorectal visceral pain,whereas the exact mechanisms remain largely unclear.In this study,we found that high expression of the 5-HT2B receptors in the Re glutamatergic neurons promoted colorectal visceral pain.Specifically,we found that neonatal maternal deprivation(NMD)mice exhibited visceral hyperalgesia and enhanced spontaneous synaptic transmission in the Re brain region.Colorectal distension(CRD)stimulation induced a large amount of c-Fos expression in the Re brain region of NMD mice,predominantly in glutamatergic neurons.Furthermore,optogenetic manipulation of glutamatergic neuronal activity in the Re altered colorectal visceral pain responses in CON and NMD mice.In addition,we demonstrated that 5-HT2B receptor expression on the Re glutamatergic neurons was upregulated and ultimately promoted colorectal visceral pain in NMD mice.These findings suggest a critical role of the 5HT2B receptors on the Re glutamatergic neurons in the regulation of colorectal visceral pain.

关 键 词：Colorectal visceral pain:Thalamic nucleus reuniens-Neonatal maternal deprivation-Glutamatergic neurons 5-HT2B receptors 

分 类 号：R338[医药卫生—人体生理学]