基于表面增强拉曼光谱技术构建水中铬价态分析试剂盒  

Construction of chromium valence analysis kit in water based on surface-enhanced Raman scattering technology

作　　者：宁琼 宋利群 张玮 秦占霞 王翠娟 NING Qiong;SONG Liqun;ZHANG Wei;QIN Zhanxia;WANG Cuijuan(Department of Occupational Disease,Shandong Academy of Occupational Health and Occupational Medicine,Shandong First Medical University&Shandong Academy of Medical Sciences,Jinan,Shandong 250062,China;Testing and Evaluation Center,Shandong Academy of Occupational Health and Occupational Medicine,Shandong First Medical University&Shandong Academy of Medical Sciences,Jinan,Shandong 250062,China;Physical and Chemical Laboratory,Shandong Academy of Occupational Health and Occupational Medicine,Shandong First Medical University&Shandong Academy of Medical Sciences,Jinan,Shandong 250062,China)

机构地区：[1]山东第一医科大学(山东省医学科学院),山东省职业卫生与职业病防治研究院职业病科,山东济南250062 [2]山东第一医科大学(山东省医学科学院),山东省职业卫生与职业病防治研究院检测评价中心,山东济南250062 [3]山东第一医科大学(山东省医学科学院),山东省职业卫生与职业病防治研究院理化分析研究室,山东济南250062

出　　处：《职业与健康》2024年第16期2188-2192,共5页Occupation and Health

基　　金：国家自然科学基金(81872575);山东省自然科学基金(ZR2023MH146,2017YL002);山东省中医药科技项目(2021M152,M-2022226);山东省医药卫生科技发展计划项目(202112070425,202115020461);山东第一医科大学学术提升计划(2017GSF18186);济南市临床医学科技创新计划项目(202225062)。

摘　　要：目的铬主要以三价铬[trivalent chromiu,Cr(Ⅲ)]和六价铬[hexavalent chromium,Cr(Ⅵ)]存在,其中Cr(Ⅵ)可对生态系统和暴露人群产生严重负面影响。本研究基于表面增强拉曼光谱(surface-enhanced Raman scattering,SERS)技术,构建水中铬价态的快速定性及定量检测试剂盒。方法采用化学合成法制备纳米银胶体溶液作为SERS活性基底,以便携式拉曼光谱仪作为检测平台,实现水中铬价态的快读分析以及Cr(Ⅵ)的现场快速定量检测,并集成为便携式试剂盒。通过优化聚沉盐种类、聚沉时间等实验条件提高灵敏度。结果本研究构建的试剂盒可以快速区分Cr(Ⅲ)和Cr(Ⅵ),检测Cr(Ⅵ)的线性范围为2μg/L~20 mg/L(R^(2)=0.997),检出限低至0.73μg/L,回收率为90.37%~97.56%,检测结果的重复性良好。结论与传统方法相比,本研究构建的铬价态分析试剂盒结果可靠,同时成本低廉、操作简便,适用于现场快速检测。Objective Chromium mainly exists in trivalent chromium[Cr(Ⅲ)]and hexavalent chromium[Cr(Ⅵ)],among which Cr(Ⅵ)can have serious negative impacts on ecosystems and exposed populations.This study is based on surface-enhanced Raman scattering(SERS)technology to construct a rapid qualitative and quantitative detection kit for the valence of chromium in water.Methods Nano silver colloidal solution was prepared by chemical synthesis as the SERS active substrate,and a portable Raman spectrometer was used as the detection platform to achieve fast reading analysis of chromium valence states in water and on-site rapid quantitative detection of Cr(Ⅵ),which were integrated into a portable reagent kit.Improve sensitivity by optimizing experimental conditions such as the type of coagulation salt and coagulation time.Results The reagent kit constructed in this study could quickly distinguish between Cr(Ⅲ)and Cr(Ⅵ),and the linear range for detecting Cr(Ⅵ)was 2μg/L to 20 mg/L(R^(2)=0.997),with a detection limit as low as 0.73μg/L,a recovery rate of 90.37%to 97.56%,demonstrating excellent reproducibility.Conclusion Compared with traditional methods,the chromium valence state analysis kit constructed in this study is low-cost,easy to operate,and suitable for rapid on-site detection.

关 键 词：表面增强拉曼光谱 铬 价态分析 便携式试剂盒 

分 类 号：R115[医药卫生—公共卫生与预防医学]