超高效液相色谱串联四级杆质谱法同时测定肉制品中11种β_(2)-受体激动剂及4种β-受体阻断剂多组分残留  

Simultaneous determination of 11 β_(2)-receptor agonists and 4β-receptor blockers in meat product based ulra-high performanee liquid chromatography tandem triplequadrupole mass speclrometry

作　　者：白应皓 栾杰 BAI Yinghao;LUAN Jie(Institute of Physical and Chemical Testing,Honghe Prefecure Center for Disease Control and Prevention,Mengzi,Yunnan 650022,China;Instiute of Physical and Chemical Testing,Yunnan Center for Disease Control and Prevention,Kunming,Yunnan 650022,China)

机构地区：[1]红河州疾病预防控制中心理化检验所,云南蒙自661100 [2]云南省疾病预防控制中心理化检验所,云南昆明650022

出　　处：《职业与健康》2024年第16期2201-2205,共5页Occupation and Health

摘　　要：目的建立一种新型、快速、准确的超高效液相色谱串联四级杆质谱检测方法,用于同时检测肉类食品中11种β_(2)-受体激动剂(妥布特罗、马布特罗、非诺特罗、奇帕特罗、喷布特罗、溴布特罗、班布特罗、莱克多巴胺、沙丁胺醇、特布他林、克仑特罗)和4种β-受体阻断剂(普萘洛尔、纳多洛尔、卡拉洛尔、阿替洛尔)。方法肉制品经5%三氯乙酸(trichloroacetic acid,TCA)提取,Waters Oasis MCX柱净化,0.1%甲酸水+10 mmol/L乙酸铵和甲醇为流动相,梯度洗脱方式,经C18柱分离,采用多重反应检测模式进行定量检测。结果11种β_(2)-受体激动剂和4种β-受体阻断剂的线性关系在0.5~20μg/L范围内表现出良好的稳定性,其相关系数r>0.9990,而且方法的加标回收率为58.5%~113.2%,相对标准偏差为0.3%~4.9%。结论所建立的方法准确、灵敏,适合于肉制品中11种β_(2)-受体激动剂和4种β-受体阻断剂的检测。Objective To establish a novel,rapid,and accurate ulra-high performance liquid chromatography tandem triplequadrupole mass speclrometry(LC-MS/MS)detection method for the simultaneous detection of 11β_(2)-receptor agonists(tobuterol,marbuterol,fenoterol,zilpaterol,pentbuterol,brobuterol,bambuterol,salbutamol,ractopamine,terbutaline,clenbuterol)and 4β-recepltor blockers(propranolol,natolol,carazololum,atenolol)in meat products.Methods The meal products were extracted by 5%trichloroacetic acid(TCA),purilied on a Waters Oasis MCX column,and the mobile phases were 0.1%formic acid water+10 mmol/L ammonium acetate.The sample were separated by Cis column by gradient elution method,and quantitated by muliplereaction detection mode.Results The linear relationship of 11β_(2)-receptor agonists and 4β-receptor blockers showed good stability at 0.5-20 mg/L,with a correlation coefficient r>0.9990.The spiked recovery rate of the method was 58.5%-113.2%,and the relative standard deviation was 0.3%-4.9%.Conclusion The established method is highly accurate,sensitive and suitable for the detection of 11β_(2)-receptor agonists and 4β-receptor blockers in meat products.

关 键 词：β_(2)-受体激动剂 Β-阻断剂 超高效液相色谱串联三重四级杆质谱法 多组分残留 

分 类 号：R115[医药卫生—公共卫生与预防医学]