荧光定量PCR和金胺O染色在结核感染中的诊断价值  

Value of fluorescence quantitative PCR and auramine O staining in tuberculosis infection diagnosis

作　　者：朱育连 齐兴峰 欧阳小娟 ZHU Yulian;QI Xingfeng;OUYANG Xiaojuan(Department of Pathology,900th Hospital of Joint Logistic Support Force,Fuzhou,Fujian 350001,China)

机构地区：[1]联勤保障部队第九〇〇医院病理科,福建福州350001

出　　处：《中国国境卫生检疫杂志》2024年第5期481-483,共3页Chinese Journal of Frontier Health and Quarantine

基　　金：福建省科技计划项目(2021Y0060);第九〇〇医院立项课题(2019L03)。

摘　　要：目的 分析荧光定量PCR和金胺O染色在结核感染中的诊断价值,提高组织切片中结核分枝杆菌的检出率。方法 选取2019年6月-2022年12月联勤保障部队第九〇〇医院收治的100例疑似结核感染患者存档蜡块为标本,分别用荧光定量PCR、金胺O染色检测结核分枝杆菌,比较两种方法的操作时间,分析单一检测和联合检测的结果及诊断效能。结果 金胺O染色操作时间(27.06±2.78)min,比荧光定量PCR短(56.56±3.62)min(P<0.05)。金胺O染色法联合荧光定量PCR的检查灵敏度为94.67%(71/75)、特异度为88.00%(22/25)、准确度为93.00%(93/100),均高于单一荧光定量PCR、金胺O染色法。经ROC曲线分析,联合检测评估结核分枝杆菌感染的预测价值(AUC=0.900)均高于荧光定量PCR (AUC=0.750)、金胺O染色法(AUC=0.722)(P<0.05)。结论 荧光定量PCR联合金胺O染色可以作为辅助方法,有助于结核感染的早期诊断。Objective To analyze the diagnostic value of quantitative fluorescence PCR and auramine O staining in tuberculosis infection,to improve the detection rate of Mycobacterium tuberculosis in tissue sections.Methods A total of 100 archived wax blocks from patients suspected of tuberculosis infection,admitted to the 900th Hospital of Joint Logistics Support Force between June 2019 and December 2022,were selected as specimens.Both quantitative fluorescence PCR and auramine O staining were employed to detect Mycobacterium tuberculosis.The operational time of each method was compared,the results along with diagnostic efficacy for single tests versus combined testing were analyzed.Results The operational time for auramine O staining was(27.06±2.78)minutes,significantly shorter than that for quantitative fluorescence PCR at(56.56±3.62)minutes(P<0.05).The sensitivity of combined auramine O staining and quantitative fluorescence PCR was found to be 94.67%(71/75),specificity was 88.00%(22/25),and accuracy reached 93.00%(93/100),all surpassing those obtained by either method alone quantitative fluorescence PCR or auramine O staining alone.ROC curve analysis indicated that the predictive value for detecting Mycobacterium tuberculosis infection using combined testing had an area under the curve(AUC)of 0.900,which was higher than that observed with quantitative fluorescence PCR(AUC=0.750)or auramine O staining alone(AUC=0.722)(P<0.05).Conclusion The combination of quantitative fluorescence PCR and auramine O staining could serve as an auxiliary method conducive to early diagnosis of tuberculosis infection.

关 键 词：荧光定量PCR 金胺O染色 结核 诊断 

分 类 号：R183.3[医药卫生—流行病学] R521[医药卫生—公共卫生与预防医学]