龙脑樟枝中一个新的桉叶烷型倍半萜内酯类化合物  

A new eudesmane-type sesquiterpenoid lactone from branches of Cinnamomum camphora chvar.Borneol

作　　者：徐志勇 肖德明 谢传奇 刘云飞 徐骏伟 李泓序 韩晓丹[1] 吴磊[1] XU Zhi-yong;XIAO De-ming;XIE Chuan-qi;LIU Yun-fei;XU Jun-wei;LI hong-xu;HAN Xiao-dan;WU Lei(Institute of Applied Chemistry,Jiangxi Academy of Sciences,Nanchang 330096,China;Jiangxi Jinggangyuan Natural Medicine Co.,Ltd.,Ji′an 331603,China)

机构地区：[1]江西省科学院应用化学研究所,南昌330096 [2]江西井冈源天然药物有限公司,吉安331603

出　　处：《天然产物研究与开发》2024年第11期1863-1867,共5页Natural Product Research and Development

基　　金：国家地区基金(22268020);江西省青年基金(20224BAB216101);江西省科学院包干制试点项目(2021YSBG22022)。

摘　　要：研究龙脑樟Cinnamomum camphora chvar.Borneol枝的化学成分。利用硅胶、Sephadex LH-20葡聚糖凝胶、MCI等柱层析,结合制备型高效液相色谱技术从龙脑樟枝乙酸乙酯萃取段中分离纯化得到3个化合物,通过波谱学数据和文献报道确定了它们的结构,分别为campholactone A(1)、northalifoline(2)、N-苯乙基-苯甲酰胺(3)。其中化合物1为新的桉叶烷型倍半萜内酯类化合物,在C-11位上存在罕见的酰胺取代,化合物2、3为首次从龙脑樟中分离得到。采用MTT法对化合物1~3进行人肝癌HepG2细胞增殖毒性评价,通过脂多糖刺激RAW 264.7小鼠巨噬细胞建立的体外炎症模型,采用Griess法研究化合物1~3对炎症介质NO生成的影响。实验结果显示化合物1~3在浓度为20μmol/L时,对肿瘤细胞HepG2表现不同程度的抑制作用,抑制率分别为35.78%±0.15%、42.36%±0.58%、23.95%±0.40%;对脂多糖诱导的RAW 264.7小鼠巨噬细胞释放炎症因子NO的抑制作用弱,抑制率小于20%。This study aims to study the chemical constituents of Cinnamomum camphora chvar.Borneol branches.Three compounds were isolated and purified from the ethyl acetate extraction of C.camphora chvar.Borneol branches by silica gel,Sephadex LH-20,MCI column chromatography and preparative high-performance liquid chromatography.Their chemical structures were identified as campholactone A(1),northalifoline(2),N-phenylethylbenzamide(3)by spectral techniques and camparison with literature data.Compound 1 is a new eucalyptus sesquiterpene lactone with a rare amide substitution at the C-11 position.Compounds 2 and 3 were isolated for the first time from titled plant.The anti-inflammatory activities of compounds 1-3 were evaluated by NO release in LPS-induced RAW 264.7 macrophages and Griess assay,the antitumor activities of compounds 1-3 against human liver cancer HepG2 cells were performed by the MTT method.The experimental results showed that compounds 1-3 exhibited varying degrees of inhibitory effects on HepG2 at a concentration of 20μmol/L,with inhibition rates of 35.78%±0.15%,42.36%±0.58%and 23.95%±0.40%,respectively;compounds 1-3 weakly inhibited the secretion of nitric oxide(NO)with an inhibition rate of less than 20%.

关 键 词：龙脑樟 桉叶烷型倍半萜内酯 化学成分 结构鉴定 

分 类 号：R284[医药卫生—中药学] R915[医药卫生—中医学]