基于原子转移自由基聚合反应的高灵敏度蛋白质印迹方法研究  

A high sensitivity Western blotting method based on atom transfer radical polymerization

作　　者：张国安 张爱红 赵利利 张艳[1] 杨晓晖[1,4] 刘颖 张晶晶[1] 孙文杰 刘英富 ZHANG Guo'an;ZHANG Aihong;ZHAO Lili;ZHANG Yan;YANG Xiaohui;LIU Ying;ZHANG Jingjing;SUN Wenjie;LIU Yingfu(Science and Technology Experiment Center,Cangzhou Medical College,Cangzhou 061001,China;Institute of Chemical Defense of PLA,Beijing 102205,China;Department of Laboratory Medicine,Fifth Medical Center(North Hospital)of Chinese PLA General Hospital,Beijing 100071,China;University Nanobody Application Technology Research and Development Center of Hebei Province,Cangzhou 061001,China;Cangzhou Nanobody Technology Innovation Center,Cangzhou 061001,China)

机构地区：[1]沧州医学高等专科学校科技实验中心,河北沧州061001 [2]陆军防化学院,北京102205 [3]解放军总医院第五医学中心(北院)检验科,北京100071 [4]河北省高校纳米抗体应用技术研发中心,河北沧州061001 [5]河北省高校沧州市纳米抗体技术创新中心,河北沧州061001

出　　处：《空军军医大学学报》2024年第11期1284-1287,共4页Journal of Air Force Medical University

基　　金：河北省重点研发计划项目(19272404D)。

摘　　要：目的探索基于金纳米颗粒(AuNPs)和原子转移自由基聚合反应(ATRP)的蛋白质印迹高灵敏度检测方法。方法将AuNPs通过ATRP附着到聚偏二氟乙烯(PVDF)膜上,通过PVDF膜对小分子的吸附作用,提高检测灵敏度。结果改进后的PVDF膜(PVDF-ATRP-AuNPs)对目标小分子的截留效率提高了80%左右,检测限显著降低。结论改进后的方法具有良好的稳定性、优异的灵敏度,能对超低浓度小分子待测物进行有效检测。Objective To explore a highly sensitive Western blotting method based on gold nanoparticles(AuNPs)and atom transfer radical polymerization(ATRP).Methods AuNPs was attached to polyvinylidene fluoride(PVDF)membranes through ATRP,and the detection sensitivity was improved by the adsorption of small molecules by PVDF membrane.Results The improved PVDF membrane(PVDF-ATRP-AuNPs)increased the interception efficiency of target small molecules by about 80%and significantly reduced the limit of detection.Conclusion The improved method has good stability and excellent sensitivity,and can effectively detect ultra-low concentration small molecule analytes.

关 键 词：蛋白质印迹法 聚偏二氟乙烯膜 纳米金 

分 类 号：Q503[生物学—生物化学] TQ316.322[化学工程—高聚物工业]