W/O乳液电纺Poloxamer188■复合聚L-乳酸纤维的表征  

Characterization of W/O Emulsion Electrospinning Poloxamer188■Composite Poly L-lactic Acid Fibers

作　　者：崔科畅 吕蕴麒 董欣 王浩 CUI Kechang;LV Yunqi;DONG Xin;WANG Hao(School of Pharmacy,Jinzhou Medical University,Jinzhou 121000 China)

机构地区：[1]锦州医科大学药学院,辽宁锦州121000

出　　处：《锦州医科大学学报》2024年第5期7-14,共8页Journal of Jinzhou Medical University

基　　金：辽宁省药物作用与质量评价专业技术创新中心与辽宁省海洋生物活性物质重点实验室主任基金项目,项目编号:2020-06,2021-4;辽宁省自然科学基金项目,项目编号:2014022041;山东省药物研究院2018年度平台开放项目,项目编号:SPKLBP201801;辽宁省大学生创新基金项目,项目编号:201710160000228。

摘　　要：目的探究Poloxamer188■复合聚乳酸W/O乳液在电纺纤维中的形态特征和材料复合性能。方法利用Poloxamer188■作为乳化剂,以Poloxamer188■和聚L-乳酸的二氯甲烷溶液作为油相,纯水作为水相,在水相与油相体积比为1∶10的条件下,通过超声波乳化技术制备出W/O型乳液,随后进行静电纺丝。通过光学显微镜观察纺丝所得产品的形态,并采用红外光谱、差示热分析、X-射线衍射和络合显色-分光光度法等技术手段,对释放出的Poloxamer188■进行测定,并绘制出时间-释放百分率曲线,以此对材料复合物进行详细表征。结果获取超细纤维,并随着Poloxamer188■复合量增加,红外光谱、差示热分析曲线和X-射线衍射图谱逐渐展现出Poloxamer188■的特征峰。Poloxamer188■从纤维中的释放表现出明显突释现象,随后是约48 h的缓释阶段,之后进入平台期。随着Poloxamer188■复合量提升,其突释量和平台期的释放量也随之增加,并呈现出线性关系。结论通过W/O乳液静电纺丝技术,可以制备出超细纤维。随着Poloxamer188■比例的调整,其与聚L-乳酸的复合程度也会相应改变。Objective To investigate the morphology and composite behavior of Poloxamer188■ polylactic acid W/O emulsion electrospinning fibers.Methods Using Poloxamer188■ as emulsifier and dichloromethane solution of Poloxamer188■and Poloxamer188■ as oil phase and pure water as water phase,W/O emulsion was prepared by ultrasonic emulsification technology under the condition of 1∶10 volume ratio of water phase to oil phase,and then electrospinning was carried out.The morphology of the spun products was observed by optical microscopy,and the released Poloxamer188■ was determined by infrared spectrums,differential thermal analysis,X-ray diffraction and complex-chromination-spectrophotometry,and the time-release percentage curve was drawn to characterize the material complex in detail.Results Ultra-fine fibers were obtained.With the increase of Poloxamer188■ composite amount,infrared spectrums,differential thermal analysis curves and X-ray diffractograms all gradually showed characteristic peaks of Poloxamer188■.The burst release of Poloxamer188■ from the fibers was considerable big followed by sustained release period of about 48 hours and then plateau period.As composite amount of Poloxamer188■ increased,burst release amounts and plateau period release amounts became bigger and bigger even presenting linear relationships.Conclusion Emulsion electrospinning yields morphologically ideal ultra-fine fibers,and composite degrees of Poloxamer188■ and poly L-lactic acid vary with the ratio of the former one.

关 键 词：Poloxamer188■ 聚L-乳酸 纤维 静电纺丝 W/O乳液 材料复合 

分 类 号：R965[医药卫生—药理学]