丛枝菌根真菌对NaCl胁迫和非胁迫下拟南芥相关指标的影响  

Effects of arbuscular mycorrhizal fungi on relative indexes of Arabidopsis thaliana under NaCl stress and non-stress

作　　者：宋娟 吴祝华[2] 王靖涵 陈凤毛[2] 闵莉静[1,2] 罗伊 SONG Juan;WU Zhuhua;WANG Jinghan;CHEN Fengmao;MIN Lijing;LUO Yi(Huzhou Normal University,Huzhou 313000,China;College of Forestry and Grassland,College of Soil and Water Conservation,Nanjing Forestry University,Nanjing 210037,China)

机构地区：[1]湖州师范学院,浙江湖州313000 [2]南京林业大学林草学院、水土保持学院,江苏南京210037

出　　处：《植物资源与环境学报》2024年第6期113-116,共4页Journal of Plant Resources and Environment

基　　金：国家自然科学基金项目(31100471)。

摘　　要：为探究丛枝菌根真菌对拟南芥〔Arabidopsis thaliana(Linn.)Heynh.〕耐盐性的影响,测定了接种摩西斗管囊霉〔Funneliformis mosseae(T.H.Nicolson et Gerd.)C.Walker et A.Schüler〕和细凹无梗囊霉(Acaulospora scrobiculata Trappe)后及(或)质量体积分数2%NaCl胁迫下拟南芥种子萌发率、幼苗鲜质量和根长及抗逆相关基因表达情况。结果显示:在无NaCl胁迫下,接种细凹无梗囊霉显著(P<0.05)提高了拟南芥种子萌发率,接种2种菌均显著提高了幼苗鲜质量而降低了幼苗根长;在NaCl胁迫下,接种2种菌均明显提高了种子萌发率及幼苗鲜质量和根长,且以细凹无梗囊霉的影响更为明显。在NaCl胁迫和非胁迫下,接种摩西斗管囊霉使PAP1、PDF1、POD表达水平显著上调,接种细凹无梗囊霉使NCED3表达水平显著上调。总体来看,摩西斗管囊霉和细凹无梗囊霉均能够促进拟南芥种子萌发和幼苗生长并提高其耐盐性,且细凹无梗囊霉的作用更为明显。To investigate the effects of arbuscular mycorrhizal fungi on salt tolerance of Arabidopsis thaliana(Linn.)Heynh.,the seed germination rate,fresh mass and root length of seedlings,and expression of stress resistance related genes in A.thaliana after inoculation with Funneliformis mosseae(T.H.Nicolson et Gerd.)C.Walker et A.Schüler and Acaulospora scrobiculata Trappe and(or)under mass volume fraction of 2%NaCl stress were measured.The results show that under no NaCl stress,inoculation with A.scrobiculata significantly(P<0.05)increases the seed germination rate of A.thaliana,while inoculations with both fungi significantly increase the fresh mass of seedlings but reduce the root length of seedlings;under NaCl stress,inoculations with both fungi significantly increase the seed germination rate and fresh mass and root length of seedlings,and the effect of A.scrobiculata is more evident.Under both NaCl stress and non-stress,inoculation with F.mosseae significantly upregulates the expression levels of PAP1,PDF1,and POD,while inoculation with A.scrobiculata significantly upregulates the expression level of NCED3.Overall,both F.mosseae and A.scrobiculata can promote the seed germination and seedling growth of A.thaliana and enhance its salt tolerance,and the effect of A.scrobiculata is more evident.

关 键 词：摩西斗管囊霉 细凹无梗囊霉 盐胁迫 拟南芥 种子萌发 幼苗生长 

分 类 号：Q948.122.3[生物学—植物学] Q948.113