侵染松墨天牛幼虫的虫生真菌鉴定及致病性测定  

Identification of entomopathogenous fungus isolated from the infected larva of Monochamus alternatus and pathogenicity test

作　　者：詹芳芳 林俨 傅世辉 林华英 林孝春 蔡守平[1] ZHAN Fangfang;LIN Yan;FU Shihui;LIN Huaying;LIN Xiaochun;CAI Shouping(Fujian Key Laboratory of Forest Cultivation and Forest Products Processing and Utilization,Fujian Academy of Forestry,Fuzhou 350012,China;Xinluo District Forestry Bureau,Longyan 364000,China;Liancheng County Forestry Bureau,Liancheng 366200,China;Shanghang County Natural Resources Bureau,Shanghang 364200,China;Fuding Forestry Bureau,Fuding 355200,China)

机构地区：[1]福建省林业科学研究院,福建省森林培育与林产品加工利用重点实验室,福建福州350012 [2]新罗区林业局,福建龙岩364000 [3]连城县林业局,福建连城366200 [4]上杭县自然资源局,福建上杭364200 [5]福鼎市林业局,福建福鼎355200

出　　处：《中国森林病虫》2024年第5期21-26,共6页Forest Pest and Disease

基　　金：2022年省属公益类科研院所专项“白僵菌固体发酵过程代谢响应的研究”(2022R1010004);2022年中央财政林业科技推广示范项目“生防真菌制剂规模化生产及防治重要林木害虫示范推广”(闽[2022]TG11号);国家林业和草原局南方山地用材林培育重点实验室资助。

摘　　要：从自然罹病的1头松墨天牛Monochamus alternatus幼虫僵虫中分离纯化得到1株虫生真菌MX-04。采用形态学和分子生物学相结合的方法对其进行鉴定,测定其生物学特性及其对松墨天牛幼虫的致病性。结果表明:菌株MX-04为粉质虫草Cordyceps farinosa,在25℃下培养14 d后产孢量为(8.39±1.03)×10^(7)个/cm^(2);菌株MX-04对松墨天牛幼虫具有较高的致病力,松墨天牛3龄幼虫接种13 d后死亡率达86.67%,LT_(50)值为6.80 d。菌株MX-04具有开发为松墨天牛生防制剂的良好潜力。The strain MX-04 of entomopathogenous fungi was isolated from the infected larva of Monochamus alternatus.Morphological and molecular biological methods were performed,and its biological characteristics and pathogenicity were studied.The results showed that strain MX-04 was identified as Cordyceps farinosa.The spore production of strain MX-04 was(8.39±1.03)×10^(7)spore/cm^(2)14 days after culture on PDA at 25℃.The C.farinosa strain MX-04 showed high virulence to the larvae of M.alternatus.The mortality of the third instar larvae of M.alternatus reached 86.67%13 days after inoculation,and the LT_(50 )value was 6.80 d.The strain MX-04 has the good potential to be developed as biocontrol agents for M.alternatus.

关 键 词：松墨天牛 粉质虫草 分离 鉴定 系统发育分析 致病力 

分 类 号：S763.306.4[农业科学—森林保护学]