高效液相色谱-二极管阵列检测器同时测定植物甾醇  

Simultaneous determination of phytosterols in medicinal materials by HPLC-DAD

作　　者：张少杰 杨定勇 文玉贵 ZHANG Shao-jie;YANG Ding-yong;WEN Yu-gui(Guangzhou University of Traditional Chinese Medicine,Guangzhou 510000,China;Hunan Drug Evaluation and Adverse Reaction Monitoring Center,Changsha 410013,China;Changsha Electronic Industry School,Changsha 410116,China)

机构地区：[1]广州中医药大学,广东广州510000 [2]湖南省药品审评与不良反应监测中心,湖南长沙410013 [3]长沙电子工业学校,湖南长沙410116

出　　处：《化学工程师》2024年第11期25-28,91,共5页Chemical Engineer

基　　金：广东省自然科学基金项目(2021A1015011736)。

摘　　要：本文建立了一种高效液相色谱-二极管阵列检测器同时测定青蒿中菜油甾醇、豆甾醇和胡萝卜苷3种植物甾醇化合物的分析方法。结果表明,该方法的精密度、准确度及线性关系良好(R^(2)>0.9994),检测限为0.55~7.07μg·mL^(-1),定量限为1.67~21.44μg·mL^(-1),日内、日间精度的相对标准偏差均小于2.93%,加样回收率为90.03%~104.91%。本文所建立的方法可成功应用于青蒿中菜油甾醇、豆甾醇和胡萝卜苷的质量控制分析。A high performance liquid chromatography-diode array detector was developed for the simultaneous determination of three phytosterols in Artemisia.The results showed that the precision,accuracy and linearity of the method were good(R^(2)>0.9994),the detection limits were 0.55~7.07μg·mL^(-1),and the limits of quantification were 1.67~21.44μg·mL^(-1).The relative standard deviations of intra-day and intra-day accuracy were both less than 2.93%.The recoveries were 90.03%~104.91%.The method established in this paper can be successfully applied to the quality control analysis of canola sterol,stigmasterol and carotenoside in Artemisia annul.

关 键 词：高效液相色谱 二极管阵列检测器 植物甾醇 青蒿 

分 类 号：R286.0[医药卫生—中药学]