三角梅自交不亲和相关基因转录组学分析  

Transcriptomics Analysis on Self-incompatibility Related Genes in Bougainvillea spp.

作　　者：杜学林 张丽华 肖子航 伍青 刘悦明 王凤兰[1] Du Xuelin;Zhang Lihua;Xiao Zihang;Wu Qing;Liu Yueming;Wang Fenglan(College of Horticulture and Landscape,Zhongkai University of Agriculture and Engineering,Guangzhou,Guangdong 510225,China;Guangzhou Institute of Forestry and Landscape Architecture,Guangzhou,Guangdong 510405,China;National Forest Germplasm Bank of Bougainvillea and Bombax ceiba L.,Guangzhou,Guangdong 510405,China)

机构地区：[1]仲恺农业工程学院园艺园林学院,广东广州510225 [2]广州市林业和园林科学研究院,广东广州510405 [3]广东省广州市簕杜鹃木棉国家林木种质资源库,广东广州510405

出　　处：《广西林业科学》2024年第5期567-576,共10页Guangxi Forestry Science

基　　金：广州市基础研究计划基础与应用基础研究项目(202201011833);省级农业科技发展及资源环境保护管理项目(2022KJ121,2023KJ121)。

摘　　要：为探究三角梅(Bougainvillea spp.)孢子体自交不亲和反应过程中相关基因的互作情况,以三角梅‘伊娃夫人’(B.glabra'Mrs.Eva')自交(♀伊娃夫人×♂伊娃夫人)和杂交([♀伊娃夫人×♂小叶紫(B.glabra'Sao paulo')]授粉不同时期(30 min、3 h和24 h)的柱头为材料,进行转录组测序,比较自交和杂交授粉在基因表达水平上的差异。结果表明,授粉后3 h和24 h,参与自交授粉反应的差异表达基因数量明显多于杂交授粉反应;自交授粉反应过程中,下调基因占主导地位;杂交授粉反应过程中,上调基因占主导地位。差异表达基因GO功能注释和富集分析结果表明,自交和杂交授粉反应过程中存在部分共同生物过程。对自交授粉不亲和相关基因(24 h)进行挖掘,筛选出32个蛋白激酶类相关基因和17个泛素化相关基因,这些基因均在自交授粉反应过程中表达上调,在杂交授粉反应过程中表达不明显。研究结果丰富了三角梅自交不亲和转录组信息,可为进一步挖掘和揭示三角梅自交不亲和机制提供依据。In order to explore interactions of related genes in process of self-incompatibility reaction of Bougainvillea spp.sporophyte,stigmas of B.glabra'Mrs.Eva'at different periods of self-pollination(♀B.glabra'Mrs.Eva'×♂B.glabra'Mrs.Eva')and cross-pollination(♀B.glabra'Mrs.Eva'×♂B.glabra'Sao paulo')(30 min,3 h and 24 h)were used as transcriptome sequencing materials to compare differences at gene expression levels between self-pollination and cross-pollination.Results showed that numbers of differentially expressed genes involved in self-pollination reaction were obviously more than those in cross-pollination reaction at 3 h and 24 h after pollination.Down-regulated genes dominated in process of self-pollination reaction,while up-regulated genes dominated in process of cross-pollination reaction.Results of GO function annotation and enrichment analysis of differentially expressed genes showed that there were some common biological processes between processes of self-pollination and cross-pollination reactions.Self-incompatibility related genes(24 h)were digged,and 32 protein kinase related genes and 17 ubiquitination related genes were screened,all of which were up-regulated in process of self-pollination reaction but not in process of cross-pollination reaction.Results of the study could enrich transcriptome information of self-incompatibility of Bougainvillea spp.and provide basis for further digging and revealing mechanisms of self-incompatibility of Bougainvillea spp..

关 键 词：三角梅 自交不亲和 转录组学 蛋白激酶 泛素化蛋白 

分 类 号：S685.99[农业科学—观赏园艺]