设计正交实验优选虎杖膏的提取工艺  

Optimization of Extraction Process for Reynoutria Japonica Cream by Orthogonal Design

作　　者：曹富 王永久 夏静 孙凡凡 Cao Fu;Wang Yongjiu;Xia Jing;Sun Fanfan(Anqing Medical College,Anqing 246052,China;Anqing Traditional Chinese Medicine Hospital,Anqing 246052,China)

机构地区：[1]安庆医药高等专科学校,安徽安庆246052 [2]安庆市中医院,安徽安庆246052

出　　处：《云南化工》2024年第11期21-23,共3页Yunnan Chemical Technology

基　　金：安徽省高校自然科学研究重点项目(2023AH053428)。

摘　　要：(目的)建立高效液相色谱法同时测定虎杖中的虎杖苷、大黄素;通过正交实验,优选虎杖膏中君药虎杖的提取工艺。(方法)采用高效液相色谱法同时测定虎杖中的虎杖苷、大黄素,以含量和浸膏得率为评价指标,考察虎杖煎煮的主要影响因素,优选水提工艺。(结果)虎杖最佳水提工艺为:8倍量水,浸泡45 min,煎煮3次,一次30 min。(结论)方法简单可行,操作性强。(Objective)High performance liquid chromatography was established to determine the content of canidin and emodin simultaneously,and the extraction process of medicine in ointment was selected through orthogonal test.(Methods)The contents of ridin and emodin were determined by HPLC,and the content and extract rate were the main influencing factors of decocting,and the water extraction technique was preferred.(Results)The best water lifting process of knotweed is:8 times water,soak for 45 min,decocting 3 times,30 min.(Conclusion)This method is simple and feasible.

关 键 词：虎杖 膏药 正交实验 高效液相色谱法 虎杖苷 大黄素 

分 类 号：TQ016[化学工程]