高效除油微生物菌株的筛选及其降解石油烃的研究  

Screening of Efficient OiL-removing Microbial Strains forthe Degradation of Petroleum Hydrocarbons

作　　者：张贝儿 曹卫承 Zhang Beier;Cao Weicheng(Shanghai pollution reduction and carbon reduction management operation technology center,Shanghai 200235,China)

机构地区：[1]上海市减污降碳管理运行技术中心,上海200235

出　　处：《云南化工》2024年第11期84-89,共6页Yunnan Chemical Technology

摘　　要：针对石油污染问题,从崇明东滩石油污染滩涂土壤中筛选出G和H两种高效降解石油烃的微生物菌株。实验显示,G、H及其联合菌剂在不同浓度石油污染土壤中均具显著降解效果,且中浓度下G+H菌剂表现最佳。对C_(6)~C_(16)液态石油烃降解稳定高效,而C_(17)~C_(36)固态石油烃在低浓度下降解显著,G菌剂尤为突出。研究发现,土壤脱氢酶活性与污染浓度及菌剂投加量密切相关,高浓度污染土壤中添加菌剂后活性提升,G+H菌剂在中浓度污染土壤中效果最佳。为石油污染滩涂生态修复提供了有效菌株和技术支持,未来可优化菌剂组合及浓度策略以提升降解效率。In this study,two microbial strains,G and H,were screened for efficient degradation of petroleum hydrocarbons in petroleum-contaminated beach soils from Chongming Dongtan.The experiments showed that G,H and their combined(G+H)bacterial agents had significant degradation effects in different concentrations of petroleum-contaminated soils,and the G+H bacterial agent had the best performance in the middle concentration.The degradation of C_(6)~C_(16)liquid petroleum hydrocarbons was stable and efficient,while the degradation of C_(17)~C_(36)solid petroleum hydrocarbons was significant at low concentrations,and the G bacterial agent was especially prominent.At the same time,it was found that soil dehydrogenase activity was closely related to the pollution concentration and the dosage of bacterial agent,and the activity was increased after adding bacterial agent in high concentration polluted soil,and G+H bacterial agent had the best effect in medium concentration polluted soil.This study provides effective strains and technical support for the ecological remediation of oiL-contaminated mudflats,and the combination and concentration of bacterial agents can be optimized in the future to enhance the degradation efficiency.

关 键 词：石油污染 微生物菌株 降解石油烃 生态修复 脱氢酶活性 

分 类 号：X53[环境科学与工程—环境工程]