黑牛肝菌多糖组分对其抗氧化能力的贡献研究  

Study on the Contribution of Polysaccharide Components ofPhlebopus portentosus to Its Antioxidant Capacity

作　　者：甘秋娅 段天昊 陈继平[1] 龚英[1] Gan Qiuya;Duan Tianhao;Chen Jiping;Gong Ying(Faculty of Chemistry and Chemical Engineering,Yunnan Normal University,Kunming 650500,China)

机构地区：[1]云南师范大学化学化工学院,云南昆明650500

出　　处：《云南化工》2024年第11期94-100,共7页Yunnan Chemical Technology

摘　　要：从黑牛肝菌中提取出的多糖可用于美容、医疗、保健等。将晾干后的黑牛肝菌进行脱脂处理,通过超声波辅助提取、传统热水提取和强碱提取,分级提取出黑牛菌中的粗多糖。对粗多糖进行脱色处理后,检测多糖含量和纯度以及单糖组成;然后采用紫外、红外和三维螺旋结构分析等方法对多糖结构进行分析;最后采用铁氰化钾显色法和水杨酸显色法对多糖和五种组成单糖标准样品对Fe^(3+)的还原力和对羟基自由基的清除率进行测定。结果表明,三步分级提取出的粗多糖含量不同,单糖种类有一定差异,但纯度都较高,都具有多糖的结构特征,都具有良好的抗氧化性能;对Fe^(3+)的还原力主要由其组分甘露糖和半乳糖贡献,但组成多糖对氧自由基的清除能力贡献不大。The polysaccharides extracted from Phlebopus portentosus can be used for beauty,medical treatment,health care,etc.After drying,the Phlebopus portentosus was degreased,and the crude polysaccharides in the Phlebopus portentosus were extracted by ultrasonic assisted extraction,traditional hot water extraction,and strong alkali extraction,and graded.After decolorizing the crude polysaccharides,the polysaccharide content,purity,and monosaccharide composition were tested;Then,the polysaccharide structure was analyzed using methods such as ultraviolet,infrared,and three-dimensional spiral structure analysis;Finally,the potassium ferrocyanide colorimetric method and salicylic acid colorimetric method were used to determine the reducing power of polysaccharides and five monosaccharide standard samples towards Fe^(3+)and the clearance rate towards hydroxyl radicals.The results showed that the crude polysaccharide content and monosaccharide types extracted by the three-step classification were different,but the purity was high and they all had the structural characteristics of polysaccharides;Both have good antioxidant properties,and the reducing power of Fe^(3+)is mainly contributed by its components,mannose and galactose,but the contribution of polysaccharides to the scavenging ability of oxygen free radicals is not significant.

关 键 词：黑牛肝菌 多糖 总抗氧化能力 

分 类 号：Q946[生物学—植物学]