β-紫罗兰酮生物合成及其关键限速酶CCDs的研究进展  

Advances on the biosynthesis ofβ-ionone and its key rate-limiting enzyme carotenoid cleavage dioxygenases

作　　者：巩效伟 吴恒 李帆 麻广慧 韩秀林 赵伟 杨柳 赵杨 段焰青 汪军 秦云华 文孟良 GONG Xiaowei;WU Heng;LI Fan;MA Guanghui;HAN Xiulin;ZHAO Wei;YANG Liu;ZHAO Yang;DUAN Yanqing;WANG Jun;QIN Yunhua;WEN Mengliang(R&D Center,China Tobacco Yunnan Industrial Co.,Ltd.,Kunming 650231,Yunnan,China;School of Life Sciences,Yunnan University,Kunming 650091,Yunnan,China;Yunnan Tobacco Industrial Hi-Tech Material Co.,Ltd.,Kunming 650106,Yunnan,China)

机构地区：[1]云南中烟工业有限责任公司技术中心,云南昆明650231 [2]云南大学生命科学学院,云南昆明650091 [3]云南中烟新材料科技有限公司,云南昆明650106

出　　处：《云南大学学报（自然科学版）》2024年第6期1159-1172,共14页Journal of Yunnan University(Natural Sciences Edition)

基　　金：国家自然科学基金(32060531);云南省基础研究专项(202101AT070279);云南省科技人才项目(202405AD350017);云南中烟工业有限责任公司科技项目(2022XY01).

摘　　要：β-紫罗兰酮是普遍存在于植物中的一种13个碳原子的萜类化合物,具有花香、果香和木香香韵,且具有抑制癌细胞增殖的活性,在食品和护理用品中应用广泛.天然β-紫罗兰酮主要通过植物提取获得,但植物中β-紫罗兰酮含量很低,难以满足市场需求.随着代谢工程和合成生物学的快速发展,β-紫罗兰酮的生物合成成为人们研究的焦点.作者主要对β-紫罗兰酮的生物合成途径、关键限速酶脱辅基类胡萝卜素裂解双加氧酶(carotenoid cleavage dioxygenases,CCDs)以及β-紫罗兰酮生物合成策略进行综述,并对当前研究的不足和未来研究方向进行了探讨,为今后以微生物为底盘的β-紫罗兰酮生物合成提供参考.β-ionone commonly present in plants is a kind of terpenoid compound with 13 carbon atoms,which has floral fragrance,as well as fruity and woody aroma characteristics.Meanwhile,β-ionone has the activity of inhibiting cancer cell proliferation.Therefore,it is widely used in food industry and care products.Nowadays,naturalβ-ionone is mainly obtained from plants extraction,but the content ofβ-ionone in plants is very low,so it is difficult to meet the needs of market.As the rapid development of metabolic engineering and synthetic biology,β-ionone biosynthesis has become the focus of people's research.This review mainly summarizesβ-ionone biosynthesis pathway,the key rate-limiting enzyme carotenoid cleavage dioxygenases(CCDs)and the strategies ofβ-ionone's biosynthesis,and discusses the shortcomings in current research and the future research direction ofβ-ionone biosynthesis,in order to provide reference for the biosynthesis ofβ-ionone based on microbial chasis in the future.

关 键 词：Β-紫罗兰酮 生物合成 类胡萝卜素裂解双加氧酶 异源表达 

分 类 号：Q503[生物学—生物化学]