Management strategies to improve the nitrogen use efficiency in grasslands and the protein quality in grass silages:Lessons from a practical study on dairy farms in Flanders  

作　　者：Tine Van den Bossche Johan LDe Boever Geert Haesaert Mathias Cougnon Sandra Debevere Leen Vandaele Karen Goossens 

机构地区：[1]ILVO,Melle,Belgium [2]Department of Plant and Crops,Ghent University,Ghent,Belgium [3]Inagro,Rumbeke-Beitem,Belgium

出　　处：《Grassland Research》2024年第1期18-32,共15页草地研究（英文）

基　　金：Agentschap Innoveren en Ondernemen,Grant/Award Number:HBC.2018.2222。

摘　　要：Background:Long-term overgrazing has led to severe degradation of grasslands,posing a significant threat to the sustainable use of grassland resources.Methods:Based on the investigation of changes in functional traits and photosynthetic physiology of Stipa breviflora under no grazing,moderate grazing,and heavy grazing treatments,the changes in expression patterns of genes and proteins associated with different grazing intensities were assessed through integrative transcriptomic and proteomic analyses.Results:Differentially expressed genes and proteins were identified under different grazing intensities.They were mainly related to RNA processing,carbon metabolism,and secondary metabolite biosynthesis.Thesefindings suggest that long-term grazing leads to molecular phenotypic plasticity,affecting various biological processes and metabolic pathways in S.breviflora.Correlation analysis revealed low correlation between the transcriptome and the proteome,indicating a large-scale regulation of gene expression at the posttranscriptional and translational levels during the response of S.breviflora to grazing.The expression profiles of key genes and proteins involved in photosynthesis and phenylpropanoid metabolism pathways suggested their synergistic response to grazing in S.breviflora.Conclusions:Our study provides insight into the adaptation mechanisms of S.breviflora to grazing and provides a scientific basis for the development of more efficient grassland protection and utilization practices.

关 键 词：CLOVER GRASSLAND grasssilage management nitrogenuseefficiency proteinquality 

分 类 号：S812[农业科学—草业科学]