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作 者:Jie Xiong Zhengxin Dong Yaru Xie Weiwen Zhang Tao Sun Lei Chen
机构地区:[1]Laboratory of Synthetic Microbiology,School of Chemical Engineering&Technology,Tianjin University,Tianjin 300072,China [2]Frontier Science Center for Synthetic Biology&Key Laboratory of Systems Bioengineering,Ministry of Education of China,Tianjin 300072,China [3]Center for Biosafety Research and Strategy,Tianjin University,Tianjin 300072,China
出 处:《Chinese Journal of Chemical Engineering》2024年第10期74-82,共9页中国化学工程学报(英文版)
基 金:supported by grants from the National Key Research and Development Programof China(2018YFA0903600);well as the National Natural Science Foundation of China(32371486 and 32270091).
摘 要:Photosynthetic cyanobacteria have shown great potential as“autotrophic cell factories”for the synthesis of fuels and chemicals.However,poor tolerance to various environmental stressors such as high light and heavy metals is an important factor limiting their economic viability.While numerous studies have focused on the tolerance mechanism of cyanobacteria to individual stressors,their response to simultaneous stresses remains to be recovered.To investigate the mechanism of cross tolerance to heavymetal Cd^(2+) and high light,the model cyanobacterium Synechocystis sp.PCC 6803 tolerant to both Cd^(2+) and high light was obtained via about 800 days’cross-adaptive laboratory evolution.Three evolutionary strains capable of tolerating both 5.5 μmol·L^(-1) Cd^(2+) and 600 μmol·m^(-2)·s^(-1) high light were successfully obtained,achieving about 83%enhancement of Cd^(2+) tolerance compared with the parent strain.The different response of parent and evolutionary strains to Cd^(2+) was elucidated via metabolomics.Furthermore,a total of 15 genes that were mutated during evolution were identified by whole-genome re-sequencing.Finally,by single-gene knockout and complementation analysis,four genes including ssl2615,sll1732,ssr1480,and sll1659 involved in the improvement of Cd^(2+) tolerance under high-light condition were successfully identified.This work explored the tolerance mechanism of Synechocystis sp.PCC 6803 to cadmium under high-light condition and provided valuable reference for deciphering multitolerance mechanism of cyanobacteria in the future.
关 键 词:SYNECHOCYSTIS Adaptive laboratory evolution Cross tolerance Cadmium-ion tolerance
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