Dragging 3D printing technique controls pore sizes of tissue engineered blood vessels to induce spontaneous cellular assembly  

作　　者：Hun-Jin Jeong Hyoryung Nam Jae-Seok Kim Sungkeon Cho Hyun-Ha Park Young-Sam Cho Hyungkook Jeon Jinah Jang Seung-Jae Lee 

机构地区：[1]Department of Mechanical Engineering,Wonkwang University,54538,Iksan,Republic of Korea [2]Department of Convergence IT Engineering,Pohang University of Science and Technology,37673,Pohang,Gyeongbuk,Republic of Korea [3]Department of Mechanical and Design Engineering,Wonkwang University,54538,Iksan,Republic of Korea [4]Department of Mechanical Engineering,Pohang University of Science and Technology,37673,Pohang,Gyeongbuk,Republic of Korea [5]School of Interdisciplinary Bioscience and Bioengineering,Pohang University of Science and Technology,37673,Pohang,Gyeongbuk,Republic of Korea [6]Institute of Convergence Science,Yonsei University,03722,Seoul,Republic of Korea [7]Regenerative Engineering Laboratory,Columbia University,630W 168th ST,New York,10032,USA [8]Department of Manufacturing Systems and Design Engineering,Seoul National University of Science and Technology,01811,Seoul,Republic of Korea

出　　处：《Bioactive Materials》2024年第1期590-602,共13页生物活性材料（英文）

基　　金：supported by the National Research Foundation of Korea(NRF)grant funded by the Korea government(MSIT)(No.2022R1A2C2008149,2021R1C1C1008767);the Korean Fund for Regenerative Medicine funded by Ministry of Science and ICT,and Ministry of Health and Welfare(21A0104L1,Republic of Korea);Korea Health Technology R&D Project through the Korea Health In-dustry Development Institute(KHIDI),funded by the Ministry of Health&Welfare,Republic of Korea(HI21C1100000021);the Alchemist Project(20012378,Development of Meta Soft Organ Module Manufacturing Technology without Immunity Rejection and Module Assembly Robot System)funded By the Ministry of Trade,Industry&Energy(MOTIE,Korea).

摘　　要：To date,several off-the-shelf products such as artificial blood vessel grafts have been reported and clinically tested for small diameter vessel(SDV)replacement.However,conventional artificial blood vessel grafts lack endothelium and,thus,are not ideal for SDV transplantation as they can cause thrombosis.In addition,a suc-cessful artificial blood vessel graft for SDV must have sufficient mechanical properties to withstand various external stresses.Here,we developed a spontaneous cellular assembly SDV(S-SDV)that develops without additional intervention.By improving the dragging 3D printing technique,SDV constructs with free-form,multilayers and controllable pore size can be fabricated at once.Then,The S-SDV filled in the natural poly-mer bioink containing human umbilical vein endothelial cells(HUVECs)and human aorta smooth muscle cells(HAoSMCs).The endothelium can be induced by migration and self-assembly of endothelial cells through pores of the SDV construct.The antiplatelet adhesion of the formed endothelium on the luminal surface was also confirmed.In addition,this S-SDV had sufficient mechanical properties(burst pressure,suture retention,leakage test)for transplantation.We believe that the S-SDV could address the challenges of conventional SDVs:notably,endothelial formation and mechanical properties.In particular,the S-SDV can be designed simply as a free-form structure with a desired pore size.Since endothelial formation through the pore is easy even in free-form con-structs,it is expected to be useful for endothelial formation in vascular structures with branch or curve shapes,and in other tubular tissues such as the esophagus.

关 键 词：Small diameter vessel Dragging 3D printing Pore control Spontaneous cellular assembly 

分 类 号：R318[医药卫生—生物医学工程]